Somatic embryogenesis in anise (Pimpinella anisum L.):: The effect of proline on embryogenic callus formation and ABA on advanced embryo development

被引:14
作者
Bela, J
Shetty, K [1 ]
机构
[1] Univ Massachusetts, Plant Biol Prog, Chenoweth Lab, Amherst, MA 01003 USA
[2] Univ Massachusetts, Dept Biol, Amherst, MA 01003 USA
关键词
D O I
10.1111/j.1745-4514.1999.tb00002.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Somatic embryos of anise (Pimpinella anisum) are an excellent system to investigate the biosynthesis of food-related phenolic metabolites like anethol which is linked to differentiation. Somatic embryogenesis in anise is a two part system. The first is a 2,4-D (2,4-dichlorophenoxy acetic acid)-induced embryogenic callus formation which requires optimal levels of endogenous auxin and cytokinin levels. The second is the advanced embryo development stage in the absence of 2,4-D which requires abscisic acid to stimulate embryo growth. Our work has shown that proline and the precursors of proline (ornithine and arginine), in combination with the proline analog, azetidine-2-carboxylate (A2C) significantly stimulated 2,4-D-induced embryogenic callus formation and subsequent somatic embryo development in hormone-free medium. Further, abscisic acid stimulated somatic embryo development in anise in the second stage. Total phenolic assays done on embryogenic tissue during 2,4-D-induced embryogenic callus formation, and during abscisic acid-mediated embryo development showed that levels were highest in embryogenic tissue induced in the presence of proline plus A2C. Total phenolic levels of developing embryos in the absence of 2,4-D and in the presence of abscisic acid were higher than that of the control. A hypothesis for further investigation is proposed based on the potential role of the proline-linked pentose phosphate pathway in stimulating the biosynthesis of purines and phenolic metabolites during anise somatic embryogenesis.
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页码:17 / 32
页数:16
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