Mutational analysis identifies a five base pair cis-acting sequence essential for GBP130 promoter activity in Plasmodium falciparum

被引：49

作者：

Horrocks, P ^{[1
]}

Lanzer, M ^{[1
]}

机构：

[1] Univ Wurzburg, Zentrum Infekt Forsch, D-97070 Wurzburg, Germany

来源：

MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 1999年 / 99卷 / 01期

关键词：

malaria; promoter; cis-acting sequence; transfection;

D O I：

10.1016/S0166-6851(98)00182-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Here we describe the functional characterization of a Plasmodium falciparum promoter region, identifying a discrete five base pair sequence element that is responsible for efficient promoter activity. This sequence element binds nuclear factors in a sequence-specific manner. It shares no homology with any known eukaryotic transcription factor binding site, supporting the notion that the protozoan parasite P. falciparum has evolved a transcriptional machinery distinct from that of its human and mosquito hosts. This report represents the first description of a minimal and necessary cis-acting sequence element for efficient promoter activity in P. falciparum. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：77 / 87

页数：11

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