Fructus Ligustri Lucidi preserves bone quality through induction of canonical Wnt/β-catenin signaling pathway in ovariectomized rats

被引：15

作者：

Liu, Haixia ^{[1
]}

Guo, Yubo ^{[1
]}

Zhu, Ruyuan ^{[1
]}

Wang, Lili ^{[1
]}

Chen, Beibei ^{[1
]}

Tian, Yimiao ^{[1
]}

Li, Rui ^{[1
]}

Ma, Rufeng ^{[1
]}

Jia, Qiangqiang ^{[1
]}

Zhang, Hao ^{[1
]}

Xia, Bingke ^{[1
]}

Li, Yu ^{[2
,3
]}

Wang, Xinxiang ^{[4
]}

Zhu, Xiaofeng ^{[5
]}

Zhang, Ronghua ^{[5
]}

Bromme, Dieter ^{[6
]}

Gao, Sihua ^{[1
]}

Zhang, Dongwei ^{[1
,3
]}

Pei, Xiaohua ^{[7
]}

机构：

[1] Beijing Univ Chinese Med, Tradit Chinese Med Sch, Diabet Res Ctr, Beijing, Peoples R China

[2] Beijing Univ Chinese Med, Tradit Chinese Med Sch, Dept Histol & Embryol, Beijing, Peoples R China

[3] Beijing Univ Chinese Med, Tradit Chinese Med Sch, Sino Canada Antifibrosis Ctr, Beijing, Peoples R China

[4] Beijing Univ Chinese Med, Affiliated Hosp 2, Ctr Expt Med, Beijing, Peoples R China

[5] Jinan Univ, Affiliated Hosp 1, Dept Chinese Med, Jinan, Peoples R China

[6] Univ British Columbia, Fac Dent, Vancouver, BC, Canada

[7] Beijing Univ Chinese Med, Fangshan Hosp BUCM, Beijing 100029, Peoples R China

来源：

PHYTOTHERAPY RESEARCH | 2021年 / 35卷 / 01期

基金：

中国国家自然科学基金;

关键词：

bone quality; Fructus Ligustri Lucidi; medicated serum; ovariectomized rats; primary osteoblasts; Wnt/beta-catenin; OSTEOBLAST DIFFERENTIATION; IGF-1; MICROARCHITECTURE; OSTEOPOROSIS; INGREDIENTS; METABOLISM; WNT;

D O I：

10.1002/ptr.6817

中图分类号：

R914 [药物化学];

学科分类号：

100705 [微生物与生化药学];

摘要：

Fructus Ligustri Lucidi(FLL) has been preclinically and clinically used to treat musculoskeletal diseases. However, whether and how FLL affect the canonical Wnt/beta-catenin signaling in the management of osteoporosis remains largely unknown. To this end, ovariectomized (OVX) rats and primary osteoblasts were administrated with FLL aqueous extract and medicated serum, respectively. Supplement of FLL to OVX rats maintains bone quality by attenuating the reduction in bone mineral density, strength and microstructure. The maintenance may be associated with upregulating the expression of insulin-like growth factor-1, osteoprotegerin, phospho (p)-low-density lipoprotein receptor-related protein 6, p-glycogen synthase kinase 3 beta (GSK3 beta), beta-catenin, Runx2 and c-Myc, and downregulating the expressions of sclerostin (SOST), dickkopf-related protein 1 (DKK1), GSK3 beta and p-beta-catenin in rat femurs and tibias. In addition, the medicated serum promotes osteoblastic bone formation through activation of Wnt/beta-catenin signaling via inhibition of DKK1 and SOST overexpression. Salidroside may be one of the active ingredients in FLL that are beneficial for bone homeostasis. In summary, our results suggest that FLL may preserve bone quality through induction of canonical Wnt/beta-catenin signaling via inhibition of DKK1 and SOST overexpression. And FLL may offer a new source of the DKK1 or SOST inhibitors in protection against osteoporosis.

引用

页码：424 / 441

页数：18

共 51 条

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