IL-6-dependent and -independent pathways in the development of interleukin 17-producing T helper cells

CD4(+) T cells producing IL-17 [T helper (Th)17], as distinct from Thl or Th2 cells, have recently been shown to be associated with autoimmunity, but it is not entirely clear how Th17 cells are generated from naive T cells. We demonstrate here that IL-6, but not TNF-alpha or IL-1 beta, can, in combination with TGF-beta, induce Th17 cell generation from naive T cells and inhibit TGF-beta-induced Foxp3 expression. Moreover, conditioned medium from lipopolysaccharide-stimulated bone marrow-derived dendritic cells (DCCM) can induce IL-17 production in naive T cells. Interestingly, IL-17 was produced by DCCM even with the addition of anti-gp 130 antibody or DCCM from IL-6 KO mice. The combination of IL-6 and TGF-P could maintain activation of signal transducer and activator of transcription (Stat)3, but not of Stat1. IL-27 or IFN-gamma suppressed the induction of Th17 cells by TGF-P plus IL-6 and maintained Stat1 activation under these conditions. In contrast, both Stat1 and Stat3 remained to be activated in naive T cells cultured with DCCM. These findings represent a different basis for Th17 differentiation from naive T cells.