Recent progress in carbohydrate separation by high-performance liquid chromatography based on size exclusion

被引：64

作者：

Churms, SC

机构：

[1] Department of Chemistry, University of Cape Town

来源：

JOURNAL OF CHROMATOGRAPHY A | 1996年 / 720卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(95)00305-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Since the introduction of stationary phases based on microparticulate porous silica and polymeric sorbents, rigid and semi-rigid, size-exclusion chromatography (SEC) has become established as a form of high-performance liquid chromatography. In recent years, there have been revolutionary developments in detection systems for highperformance SEC, which have placed the use of the method for the determination of molecular-size and molecular-weight distributions of polymers on a sound theoretical basis and increased the range of information on molecular characteristics that can be retrieved from SEC data. This review surveys these changes in SEC systems and their application to the separation and molecular-weight distribution analysis of carbohydrates.

引用

页码：151 / 166

页数：16

共 70 条

[1] AGAROSE-BASED MEDIA FOR HIGH-RESOLUTION GEL-FILTRATION OF BIO-POLYMERS [J].