Identification of a receptor tyrosine kinase involved in germ cell differentiation in planarians

被引:29
作者
Ogawa, K
Wakayama, A
Kunisada, T
Orii, H
Watanabe, K
Agata, K [1 ]
机构
[1] Himeji Inst Technol, Fac Sci, Dept Life Sci, Lab Regenerat Biol, Akou, Hyogo 6781297, Japan
[2] Tottori Univ, Fac Med, Sch Life Sci, Dept Immunol, Yonago, Tottori 683, Japan
关键词
protein tyrosine kinase; receptor; planarian; Dugesia japonica; germ cell;
D O I
10.1006/bbrc.1998.8915
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
To investigate external signals involved in germ cell differentiation from somatic stem cells, we have tried to identify protein kinases whose expression is regulated during the process of sexualization of asexual-state planarians. It is known that in planarians germ cells differentiate from totipotent somatic stem cells called "neoblasts" during sexualization. As a first step, we have isolated twelve protein kinase genes from cDNAs of sexual-state planarians, including three non-receptor tyrosine kinases, three receptor-tyrosine kinases and three non-receptor serine/threonine kinases, and then analyzed their expression patterns during sexualization. One of them, the DjPTK1 gene, is specifically expressed in germ cells of sexual-state planarians. DjPTK1-positive cells were also detected in the mesenchymal space during the process of sexualization, and it appears that these cells migrate to the dorsal side and then differentiate into spermatogonia/spermatocytes in testis. Sequence analysis indicated that the DjPTK1 gene encodes a receptor protein tyrosine kinase belonging to the FGFR/PDGF family. These results suggest that a receptor tyrosine kinase system may be involved both at an early stage of germ cell differentiation and in a step of germ cell maturation in planarians. (C) 1998 Academic Press.
引用
收藏
页码:204 / 209
页数:6
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