Significant reduction of proinflammatory cytokines by treatment of the brain-dead donor

被引:54
作者
Kuecuek, O
Mantouvalou, L
Klemz, R
Kotsch, K
Volk, HD
Jonas, S
Wesslau, C
Tullius, S
Neuhaus, P
Pratschke, J
机构
[1] Univ Med Berlin, Charite, Dept Surg, D-13353 Berlin, Germany
[2] DSO Berlin Brandenburg, Berlin, Germany
[3] Univ Regensburg, Regensburg, Germany
关键词
D O I
10.1016/j.transproceed.2004.12.165
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Introduction. Experimental studies suggest that brain death in the donor has a significant impact on graft quality; however, there are no data correlating organ-specific cytokine expression and the corresponding serum protein levels in human organ donors. Furthermore, it is unknown whether donor treatment can reduce the up-regulation of proinflammatory cytokines and thereby optimize organ quality. Methods. We investigated the expression pattern of cytokines comparing serum (n = 53) and tissue expression (n = 25) in brain-dead human donors. The controls were living donors (n = 25). Additionally 41 deceased donors were treated with steroids before organ harvest (250 mg initial, afterward 100 mg/h until laparotomy). Hepatic tissue samples were obtained immediately after donor laparotomy to assess transcription rates of tissue cytokines (IL-6, IL-10, CD3, TGFb, TNFa, BAG, HO-1, Mipla) by RT-PCR. Serum samples were obtained after declaration of brain death and before laparotomy. Results. Transcription of proinflammatory cytokines was significantly increased in brain-dead compared to living donor grafts (P < .005). Donor treatment with steroids led to significantly decreased tissue and serum expression of proinflammatory cytokines (P < .01), which were comparable to living donors. Tissue levels of cytokines (IL-6, IL-10) correlated strongly with serum levels of the corresponding proteins. Conclusions. Serum protein levels of proinflammatory cytokines proffer a valuable, easy accessible marker to define the immunological status of a graft. Our data suggest a beneficial effect of anti-inflammatory treatment of brain-dead organ donors.
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页码:387 / 388
页数:2
相关论文
共 5 条
[1]  
CECKA JM, 2000, CLIN TRANSPL, V1
[2]   Cadaveric versus living-donor livers: Differences in inflammatory markers after transplantation [J].
Jassem, W ;
Koo, DDH ;
Cerundolo, L ;
Rela, M ;
Heaton, ND ;
Fuggle, SV .
TRANSPLANTATION, 2003, 76 (11) :1599-1603
[3]   Leukocyte infiltration and inflammatory antigen expression in cadaveric and living-donor livers before transplant. [J].
Jassem, W ;
Koo, DDH ;
Cerundolo, L ;
Rela, M ;
Heaton, ND ;
Fuggle, SV .
TRANSPLANTATION, 2003, 75 (12) :2001-2007
[4]   Accelerated rejection of renal allografts from brain-dead donors [J].
Pratschke, J ;
Wilhelm, MJ ;
Kusaka, M ;
Beato, F ;
Milford, EL ;
Hancock, WW ;
Tilney, NL .
ANNALS OF SURGERY, 2000, 232 (02) :263-271
[5]   Activation of the heart by donor brain death accelerates acute rejection after transplantation [J].
Wilhelm, MJ ;
Pratschke, J ;
Beato, F ;
Taal, M ;
Kusaka, M ;
Hancock, WW ;
Tilney, NL .
CIRCULATION, 2000, 102 (19) :2426-2433