A poly(o-aminophenol) modified electrode as an amperometric hydrogen peroxide biosensor

被引：52

作者：

Garcia, MAV

Blanco, PT ^{[1
]}

Ivaska, A

机构：

[1] Univ Oviedo, Dept Quim Fis & Analit, Oviedo 33006, Asturias, Spain

[2] Abo Akad Univ, Analyt Chem Lab, SF-20500 Turku, Finland

来源：

ELECTROCHIMICA ACTA | 1998年 / 43卷 / 23期

关键词：

enzymatic biosensor; hydrogen peroxide; poly(o-aminophenol) films; amperometric; flow injection analysis;

D O I：

10.1016/S0013-4686(98)00101-7

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

An enzymatic biosensor for hydrogen peroxide based on a horseradish peroxidase (HRP)-ferrocene carbon paste modified electrode and coated with a layer of electrochemically generated poly(o-aminophenol) is reported. A linear calibration curve is obtained over the range 1 x 10(-8) M to 1 x 10(-5) M. The biosensor responds to hydrogen peroxide ina few seconds and has a detection limit of 8.5 x 10(-9) M. The response of the biosensor is diffusion controlled at low substrate concentrations. Flow injection assays of hydrogen peroxide at a sampling rate of 150 injections per hour with a relative standard deviation of 0.8% (50 samples) are possible. Applicability of the sensor for measurement of hydrogen peroxide in real samples (milk) was demonstrated. (C) 1998 Elsevier Science Ltd. All rights reserved.

引用

页码：3533 / 3539

页数：7

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