Isolation and characterization of polysaccharides from Agaricus blazei Murill

被引:129
作者
Gonzaga, MLC
Ricardo, NMPS
Heatley, F
Soares, SD
机构
[1] Univ Fed Ceara, Dept Quim Organ & Inorgan, Lab Polimeros, BR-60455760 Fortaleza, Ceara, Brazil
[2] Univ Manchester, Dept Chem, Manchester M13 9PL, Lancs, England
关键词
Agaricus blazei Murill; polysaccharides; FTIR; NMR;
D O I
10.1016/j.carbpol.2004.11.022
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
An efficient route has been developed to isolate water soluble polysaccharides from Agaricus blazei Murill mushroom of Brazilian origin. Using different temperatures: 10, 60 and 100 degrees C, and precipitation with ethanol, a number of precipitates were extracted which were isolated, purified and fractionated for characterization. The molar mass distribution for the precipitates and fractions from the extraction at 100 degrees C were determined by GPC. Analysis of the FTIR, H-1 NMR and C-13 NMR spectra showed a pattern characteristic of glucan-protein complex. (c) 2004 Elsevier Ltd. All rights reserved.
引用
收藏
页码:43 / 49
页数:7
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