Identification of grapevines infected with divergent variants of Grapevine virus A using variant-specific RT-PCR

被引：22

作者：

Goszczynski, DE ^{[1
]}

Jooste, AEC ^{[1
]}

机构：

[1] Agr Res Council, Res Inst Plant Protect, ZA-0001 Pretoria, South Africa

来源：

JOURNAL OF VIROLOGICAL METHODS | 2003年 / 112卷 / 1-2期

关键词：

grapevines; N; benthamiana; GVA variants; variant-specific PCR; SSCP; cloning and sequencing;

D O I：

10.1016/S0166-0934(03)00198-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

RT-PCRs. designed for the specific detection of molecular variants of Grapevine virus A (GVA), were applied to analysis of the virus from various grapevines and isolates recovered from these grapevines in Nicotiana benthamiana. Results of SSCP, cloning and sequencing revealed that the combination of these RT-PCR techniques permits reliable and rapid identification of grapevines mixed-infected with divergent variants of GVA. The results suggest that such grapevines are very common among GVA-infected grapevines in vineyards in South Africa. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：157 / 164

页数：8

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