A quantitative and qualitative method for direct 2-DE analysis of murine cartilage

被引：19

作者：

Pecora, Fabio

Forlino, Antonella

Gualeni, Benedetta

Lupi, Anna

Giorgetti, Sofia

Marchese, Loredana

Stoppini, Monica

Tenni, Ruggero

Cetta, Giuseppe

Rossi, Antonio

机构：

[1] Univ Pavia, Dipartimento Biochim Alessandro Castellani, I-27100 Pavia, Italy

[2] IRCCS Policlin S Matteo, Lab Biotecnol, Pavia, Italy

来源：

PROTEOMICS | 2007年 / 7卷 / 21期

关键词：

2-DE optimization; articular cartilage; mouse; proteoglycans;

D O I：

10.1002/pmic.200700276

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Direct 2-DE analysis of cartilage is difficult due to the high proteoglycan content. Proteoglycan removal before IEF may however cause the partial or total loss of specific proteins making this approach ineffective when quantitative data are required to investigate protein expression differences. Thus, we have developed a 2-DE method including passive rehydration loading that does not require sample pretreatment and allows direct protein expression studies in cartilage samples.

引用

收藏

页码：4003 / 4007

页数：5

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