Antitumor activity of cytokine-induced killer cells against human lung cancer

被引：113

作者：

Kim, Hwan Mook ^{[2
]}

Lim, Jaeseung ^{[3
]}

Park, Song-Kyu ^{[2
]}

Kang, Jong Soon ^{[2
]}

Lee, Kiho ^{[2
]}

Lee, Chang Woo ^{[2
]}

Lee, Ki Hoon ^{[2
]}

Yun, Mi-Jung ^{[3
]}

Yang, Kyu-Hwan ^{[4
]}

Han, Gyoonhee ^{[5
]}

Kwon, Soon Woo ^{[1
]}

Kim, Youngsoo ^{[1
]}

Han, Sang-Bae ^{[1
]}

机构：

[1] Chungbuk Natl Univ, Coll Pharm, Cheongju 361763, Chungbuk, South Korea

[2] KRIBB, Bioevaluat Ctr, Ochang 363883, Chungbuk, South Korea

[3] Innocell Ltd, Seoul 153769, South Korea

[4] Kyungwon Univ, Songnam 461701, Gyeonggi, South Korea

[5] Yonsei Univ, Dept Biotechnol, Seoul 120740, South Korea

来源：

INTERNATIONAL IMMUNOPHARMACOLOGY | 2007年 / 7卷 / 13期

基金：

新加坡国家研究基金会;

关键词：

cytokine-induced killer cells; lung cancer; adoptive immunotherapy;

D O I：

10.1016/j.intimp.2007.08.016

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 [免疫学];

摘要：

Lung cancer is the leading cause of cancer-related death among men and women in the world. Despite the aggressive treatment with surgery, radiation and chemotherapy, the long term survival for lung cancer patients remains low. In this study, the anti-tumor activity of cytokine-induced killer (CIK) cells against human lung cancer was evaluated in vitro and in vivo. Although CD3(+)CD56(+) CIK cells were rare in fresh human peripheral blood mononuclear cells, they could expand more than 1000-fold on day 14 in the presence of anti-CD3 antibody plus IL-2. At an effector-target cell ratio of 30:1, CIK cells destroyed 98% of NCI-H460 human lung cancer cells, which was determined by the Cr-51-release assay. In addition, CIK cells at doses of 3 and 30 million cells per mouse inhibited 57% and 77% of NCI-H460 tumor growth in nude mouse xenograft assay, respectively. This study suggests that CD3+CD56+ CIK cells may be used as an adoptive immunotherapy for patients with lung cancer. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：1802 / 1807

页数：6

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