The proteome of Saccharomyces cerevisiae mitochondria

被引：694

作者：

Sickmann, A

Reinders, J

Wagner, Y

Joppich, C

Zahedi, R

Meyer, HE

Schönfisch, B

Perschil, I

Chacinska, A

Guiard, B

Rehling, P

Pfanner, N

Meisinger, C

机构：

[1] Univ Freiburg, Inst Biochem & Mol Biol, D-79104 Freiburg, Germany

[2] Univ Wurzburg, Rudolf Virchow Ctr Expt Biomed, D-97078 Wurzburg, Germany

[3] Ruhr Univ Bochum, Med Proteom Ctr, D-44780 Bochum, Germany

[4] Univ Paris 06, CNRS, Lab Propre, Ctr Genet Mol, F-91190 Gif Sur Yvette, France

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2003年 / 100卷 / 23期

关键词：

D O I：

10.1073/pnas.2135385100

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We performed a comprehensive approach to determine the proteome of Saccharomyces cerevisiae mitochondria. The proteins of highly pure yeast mitochondria were separated by several independent methods and analyzed by tandem MS. From >20 million MS spectra, 750 different proteins were identified, indicating an involvement of mitochondria in numerous cellular processes. All known components of the oxidative phosphorylation machinery, the tricarboxylic acid cycle, and the stable mitochondria-encoded proteins were found. Based on the mitochondrial proteins described in the literature so far, we calculate that the identified proteins represent approximate to90% of all mitochondrial proteins. The function of a quarter of the identified proteins is unknown. The mitochondrial proteome will provide an important database for the analysis of new mitochondrial and mitochondria-associated functions and the characterization of mitochondrial diseases.

引用

页码：13207 / 13212

页数：6

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