Inositolphosphoceramide is not a substrate for the first steps in the biosynthesis of glycoinositolphospholipids in Trypanosoma cruzi

被引:14
作者
Bertello, LE
Alves, MJM
Colli, W
de Lederkremer, RM
机构
[1] Univ Buenos Aires, Fac Ciencias Exactas & Nat, Dept Quim Organ, CIHIDECAR, RA-1428 Buenos Aires, DF, Argentina
[2] Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-05599970 Sao Paulo, Brazil
基金
巴西圣保罗研究基金会;
关键词
T cruzi; ceramide; inositolphosphoceramide; GPI anchors; glycoinositolphospholipids;
D O I
10.1016/j.molbiopara.2003.09.007
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The major free glycoinositolphospholipids and protein-linked glycoinositolphospholipids in Trypanosoma cruzi contain ceramide as the lipid moiety. Ceramide was not found in mammalian glycosylphosphatidylinositol (GPI)-anchors. An alkylglycerol, either as a lyso species or acylated has been also found in T cruzi anchors. However, unlike African trypanosomes, no diacylglycerol was detected in the GPI-anchors. Using a membrane preparation from. epimastigotes upon labelling with UDP[H-3]GlcNAc we identified [H-3]GlcNAcPI as the first step of GPI biosynthesis. Both, alkylacylglycerol (major) and diacylglycerol are constituents of the lipid. Although inositolphosphoceramide is the main inositolphospholipid in epimastigotes, it does not incorporate GlcNAc. The de-N-acetylation step afforded [H-3]GlcN(alkylacylglycerol)PI and we also detected the [H-3]GlcN(lysoacyl)PI. A new metabolite, phosphoGlcN(lysoacyl)PI, which was formed on long incorporation times, was characterized by chemical and enzymatic degradations. Several [H-3]-Man labelled GPI precursors were obtained by in vitro GDP[H-3]-Man labelling in the presence of UDPGlcNAc. All of them were sensitive to PI-PLC and to saponification conditions, thus, supporting a glycerolipid structure. (C) 2003 Elsevier B.V. All rights reserved.
引用
收藏
页码:71 / 80
页数:10
相关论文
共 33 条
[1]   Trypanosoma cruzi: The Tc-85 surface glycoprotein shed by trypomastigotes bears a modified glycosylphosphatidylinositol anchor [J].
Abuin, G ;
Couto, AS ;
DeLederkremer, RM ;
Casal, OL ;
Galli, C ;
Colli, W ;
Alves, MJM .
EXPERIMENTAL PARASITOLOGY, 1996, 82 (03) :290-297
[2]  
Acosta-Serrano A., 1995, J BIOL CHEM, V270, P27244
[3]   The trans-sialidase of Trypanosoma cruzi is anchored by two different lipids [J].
Agusti, R ;
Couto, AS ;
Campetella, OE ;
Frasch, ACC ;
deLederkremer, RM .
GLYCOBIOLOGY, 1997, 7 (06) :731-735
[4]   Structure of the glycosylphosphatidylinositol-anchor of the trans-sialidase from Trypanosoma cruzi metacyclic trypomastigote forms [J].
Agusti, R ;
Couto, AS ;
Campetella, O ;
Frasch, ACC ;
de Lederkremer, RM .
MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1998, 97 (1-2) :123-131
[5]   Developmentally regulated expression of ceramide in Trypanosoma cruzi [J].
Bertello, LE ;
Andrews, NW ;
deLederkremer, RM .
MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1996, 79 (02) :143-151
[6]   STRUCTURAL-ANALYSIS OF INOSITOL PHOSPHOLIPIDS FROM TRYPANOSOMA-CRUZI EPIMASTIGOTE FORMS [J].
BERTELLO, LE ;
GONCALVEZ, MF ;
COLLI, W ;
DELEDERKREMER, RM .
BIOCHEMICAL JOURNAL, 1995, 310 :255-261
[7]   Evidence for phospholipases from Trypanosoma cruzi active on phosphatidylinositol and inositolphosphoceramide [J].
Bertello, LE ;
Alves, MJM ;
Colli, W ;
de Lederkremer, RM .
BIOCHEMICAL JOURNAL, 2000, 345 :77-84
[8]  
Camargo MM, 1997, J IMMUNOL, V158, P5890
[9]   INVITRO DIFFERENTIATION OF TRYPANOSOMA-CRUZI UNDER CHEMICALLY DEFINED CONDITIONS [J].
CONTRERAS, VT ;
SALLES, JM ;
THOMAS, N ;
MOREL, CM ;
GOLDENBERG, S .
MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1985, 16 (03) :315-327
[10]   THE GLYCOSYLPHOSPHATIDYLINOSITOL ANCHOR OF THE TRYPOMASTIGOTE-SPECIFIC TC-85 GLYCOPROTEIN FROM TRYPANOSOMA-CRUZI - METABOLIC-LABELING AND STRUCTURAL STUDIES [J].
COUTO, AS ;
DELEDERKREMER, RM ;
COLLI, W ;
ALVES, MJM .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1993, 217 (02) :597-602