Expression of a functional barley sucrose-fructan 6-fructosyltransferase in the methylotrophic yeast Pichia pastoris

被引:45
作者
Hochstrasser, U [1 ]
Lüscher, M [1 ]
De Virgilio, C [1 ]
Boller, T [1 ]
Wiemken, A [1 ]
机构
[1] Univ Basel, Dept Bot, CH-4056 Basel, Switzerland
关键词
fructan; heterologous expression; sucrose-fructan; 6-fructosyltransferase; Hordeum vulgare; Pichia pastoris;
D O I
10.1016/S0014-5793(98)01487-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The cDNA encoding sucrose-fructan 6-fructosyltransferase (6-SFT) from barley (Hordeum vulgare) has been expressed in the methylotrophic yeast Pichia pastoris, using a translational fusion into vector pPICZ alpha C, containing the N-terminal signal sequence of Saccharomyces cerevisiae alpha-factor to allow entry into the secretory: pathway. Transformed Pichia produced and secreted a functional 6-SFT which had characteristics similar to the barley enzyme, but had a pronounced additional 1-SST activity when incubated with sucrose, (C) 1998 Federation of European Biochemical Societies.
引用
收藏
页码:356 / 360
页数:5
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