Expression of a functional barley sucrose-fructan 6-fructosyltransferase in the methylotrophic yeast Pichia pastoris

被引：45

作者：

Hochstrasser, U ^{[1
]}

Lüscher, M ^{[1
]}

De Virgilio, C ^{[1
]}

Boller, T ^{[1
]}

Wiemken, A ^{[1
]}

机构：

[1] Univ Basel, Dept Bot, CH-4056 Basel, Switzerland

来源：

FEBS LETTERS | 1998年 / 440卷 / 03期

关键词：

fructan; heterologous expression; sucrose-fructan; 6-fructosyltransferase; Hordeum vulgare; Pichia pastoris;

D O I：

10.1016/S0014-5793(98)01487-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The cDNA encoding sucrose-fructan 6-fructosyltransferase (6-SFT) from barley (Hordeum vulgare) has been expressed in the methylotrophic yeast Pichia pastoris, using a translational fusion into vector pPICZ alpha C, containing the N-terminal signal sequence of Saccharomyces cerevisiae alpha-factor to allow entry into the secretory: pathway. Transformed Pichia produced and secreted a functional 6-SFT which had characteristics similar to the barley enzyme, but had a pronounced additional 1-SST activity when incubated with sucrose, (C) 1998 Federation of European Biochemical Societies.

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收藏

页码：356 / 360

页数：5

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