Atomic structure of the clamp loader small subunit from Pyrococcus furiosus

被引:64
作者
Oyama, T
Ishino, Y
Cann, IKO
Ishino, S
Morikawa, K
机构
[1] Biolmol Engn Res Inst, Dept Biol Struct, Suita, Osaka 5650874, Japan
[2] Biolmol Engn Res Inst, Dept Mol Biol, Suita, Osaka 5650874, Japan
关键词
D O I
10.1016/S1097-2765(01)00328-8
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In eukaryotic DNA replication, replication factor-C (RFC) acts as the clamp loader, which correctly installs the sliding clamp onto DNA strands at replication forks. The eukaryotic RFC is a complex consisting of one large and four small subunits. We have determined the crystal structure of the clamp loader small subunit (RFCS) from Pyrococcus furiosus. The six subunits, of which four bind ADP in their canonical nucleotide binding clefts, assemble into a dimer of semicircular trimers. The crescent-like architecture of each subunit formed by the three domains resembles that of the delta' subunit of the E. coli clamp loader. The trimeric architecture of archaeal RFCS, with its mobile N-terminal domains, involves intersubunit interactions that may be conserved in eukaryotic functional complexes.
引用
收藏
页码:455 / 463
页数:9
相关论文
共 40 条
[1]   THE CCP4 SUITE - PROGRAMS FOR PROTEIN CRYSTALLOGRAPHY [J].
BAILEY, S .
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 1994, 50 :760-763
[2]   3D DOMAIN SWAPPING - A MECHANISM FOR OLIGOMER ASSEMBLY [J].
BENNETT, MJ ;
SCHLUNEGGER, MP ;
EISENBERG, D .
PROTEIN SCIENCE, 1995, 4 (12) :2455-2468
[3]   The structures of HsIU and ATP-dependent protease HsIU-HsIV [J].
Bochtler, M ;
Hartmann, C ;
Song, HK ;
Bourenkov, GP ;
Bartunik, HD ;
Huber, R .
NATURE, 2000, 403 (6771) :800-805
[4]   Crystallography & NMR system:: A new software suite for macromolecular structure determination [J].
Brunger, AT ;
Adams, PD ;
Clore, GM ;
DeLano, WL ;
Gros, P ;
Grosse-Kunstleve, RW ;
Jiang, JS ;
Kuszewski, J ;
Nilges, M ;
Pannu, NS ;
Read, RJ ;
Rice, LM ;
Simonson, T ;
Warren, GL .
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 1998, 54 :905-921
[5]   A complex consisting of human replication factor C p40, p37, and p36 subunits is a DNA-dependent ATPase and an intermediate in the assembly of the holoenzyme [J].
Cai, JS ;
Gibbs, E ;
Uhlmann, F ;
Phillips, B ;
Yao, N ;
ODonnell, M ;
Hurwitz, J .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (30) :18974-18981
[6]   Biochemical analysis of replication factor C from the hyperthermophilic archaeon Pyrococcus furiosus [J].
Cann, IKO ;
Ishino, S ;
Yuasa, M ;
Daiyasu, H ;
Toh, H ;
Ishino, Y .
JOURNAL OF BACTERIOLOGY, 2001, 183 (08) :2614-2623
[7]  
Cann IKO, 1999, GENETICS, V152, P1249
[8]  
CULLMANN G, 1995, MOL CELL BIOL, V15, P4661
[9]   Maximum-likelihood heavy-atom parameter refinement for multiple isomorphous replacement and multiwavelength anomalous diffraction methods [J].
delaFortelle, E ;
Bricogne, G .
MACROMOLECULAR CRYSTALLOGRAPHY, PT A, 1997, 276 :472-494
[10]   Archaea and the origin(s) of DNA replication proteins [J].
Edgell, DR ;
Doolittle, WF .
CELL, 1997, 89 (07) :995-998