Growth hormone stimulates androsterone synthesis by rat theca-interstitial cells

被引：23

作者：

Apa, R ^{[1
]}

Caruso, A ^{[1
]}

Andreani, CL ^{[1
]}

Miceli, F ^{[1
]}

Lazzarin, N ^{[1
]}

Mastrandrea, M ^{[1
]}

Ronsisvalle, E ^{[1
]}

Mancuso, S ^{[1
]}

Lanzone, A ^{[1
]}

机构：

[1] IST OASI, TROINA, ITALY

来源：

MOLECULAR AND CELLULAR ENDOCRINOLOGY | 1996年 / 118卷 / 1-2期

关键词：

growth hormone; androsterone; insulin growth factor-I; rat theca-interstitial cells;

D O I：

10.1016/0303-7207(96)03769-0

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The aim of this study was to assess the possible role of growth hormone (GH) on androsterone synthesis. This effect was analyzed in theca-interstitial cells obtained from immature female rats. The addition of GH to the cultures significantly stimulated androsterone (A) synthesis in a dose- and time-dependent way and this effect was not due to a cellular number increase. When added to the hCG cultures, GH significantly enhanced androgen production even though it did not synergyze with the chorionic gonadotropin. The addition of antibodies anti-IGF-I to the GH cultures did not modify the growth hormone effect suggesting that GH probably does not require IGF-I to achieve its effect on A production. Finally, no effect of GH on cAMP levels were observed in the cultures at the end of the treatment. Our results demonstrate that GH is able to significantly induce A synthesis by rat theca-interstitial cells. Since the presence of GH and its receptors in the ovary is now well established the present data strongly suggest a potential relevance of GH in reproductive biology.

引用

页码：95 / 101

页数：7

共 40 条

[1] INDEPENDENT AND SYNERGISTIC ACTIONS OF SOMATOMEDIN-C IN THE STIMULATION OF PROTEOGLYCAN BIOSYNTHESIS BY CULTURED RAT GRANULOSA-CELLS [J].