Crystal structure of the soluble form of equinatoxin II, a pore-forming toxin from the sea anemone Actinia equina

被引:178
作者
Athanasiadis, A
Anderluh, G
Macek, P
Turk, D
机构
[1] Jozef Stefan Inst, Dept Biochem & Mol Biol, Ljubljana 1000, Slovenia
[2] Univ Ljubljana, Biotech Fac, Dept Biol, Ljubljana 1000, Slovenia
关键词
actinoporin; cathion channel; equinatoxin II; membrane pore; crystal structure; soluble form;
D O I
10.1016/S0969-2126(01)00592-5
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Background: Membrane pore-forming toxins have a remarkable property: they adopt a stable soluble form structure, which, when in contact with a membrane, undergoes a series of transformations, leading to an active, membrane-bound form. In contrast to bacterial toxins, no structure of a pore-forming toxin from an eukaryotic organism has been determined so far, an indication that structural studies of equinatoxin II (EqtII) may unravel a novel mechanism. Results: The crystal structure of the soluble form of EqtII from the sea anemone Actinia equina has been determined at 1.9 Angstrom resolution. EqtII is shown to be a single-domain protein based on a 12 strand beta sandwich fold with a hydrophobic core and a pair of ct helices, each of which is associated with the face of a 13 sheet. Conclusions: The structure of the 30 N-terminal residues is the largest segment that can adopt a different structure without disrupting the fold of the beta sandwich core. This segment includes a three-turn a helix that lies on the surface of a beta sheet and ends in a stretch of three positively charged residues, Lys-30, Arg-31, and Lys-32. On the basis of gathered data, it is suggested that this segment forms the membrane pore, whereas the beta sandwich structure remains unaltered and attaches to a membrane as do other structurally related extrinsic membrane proteins or their domains. The use of a structural data site-directed mutagenesis study should reveal the residues involved in membrane pore formation.
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收藏
页码:341 / 346
页数:6
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