Surface-enhanced Raman scattering substrate based on a self-assembled monolayer for use in gene diagnostics

被引：150

作者：

Culha, M ^{[1
]}

Stokes, D ^{[1
]}

Allain, LR ^{[1
]}

Vo-Dinh, T ^{[1
]}

机构：

[1] Oak Ridge Natl Lab, Adv Biomed Sci & Technol Grp, Oak Ridge, TN 37831 USA

来源：

ANALYTICAL CHEMISTRY | 2003年 / 75卷 / 22期

关键词：

D O I：

10.1021/ac0346003

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The development of surface-enhanced Raman scattering (SERS)-active substrates for cancer gene detection is described. The detection method uses Raman active dye-labeled DNA gene probes, self-assembled monolayers, and nanostructured metallic substrates as SERS-active platforms. The mercaptohexane-labeled single-stranded DNA (SH - (CH2)(6)-ssDNA)/6-mercapto-1-hexanol system formed on a silver surface is characterized by atomic force microcopy. The surface-enhanced Raman gene (SERGen) probes developed in this study can be used to detect DNA targets via hybridization to complementary DNA probes. The probes do not require the use of radioactive labels and have a great potential to provide both sensitivity and selectivity. The effectiveness of this approach and its application in cancer gene diagnostics (BRCA1 breast cancer gene) are investigated.

引用

页码：6196 / 6201

页数：6

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