Production, purification and preliminary X-ray crystallographic studies of adeno-associated virus serotype 7

被引：16

作者：

Quesada, Odayme ^{[1
]}

Gurda, Brittney ^{[1
]}

Govindasamy, Lakshmanan ^{[1
]}

McKenna, Robert ^{[1
]}

Kohlbrenner, Erik ^{[2
]}

Aslanidi, George

Zolotukhin, Sergei ^{[2
]}

Muzyczka, Nicholas ^{[3
]}

Agbandje-McKenna, Mavis ^{[1
]}

机构：

[1] Univ Florida, McKnight Brain Inst, Ctr Struct Biol, Dept Biochem & Mol Biol, Gainesville, FL 32610 USA

[2] Univ Florida, Dept Pediat, Div Cell & Mol Theraphy, Gainesville, FL 32610 USA

[3] Univ Florida, Coll Med, Powell Gene Theraphy Ctr, Dept Mol Genet & Microbiol, Gainesville, FL 32610 USA

来源：

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2007年 / 63卷

关键词：

D O I：

10.1107/S1744309107060289

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Crystals of baculovirus-expressed adeno-associated virus serotype 7 capsids diffract X-rays to similar to 3.0 angstrom resolution. The crystals belong to the rhombohedral space group R3, with unit-cell parameters a = 252.4, c = 591.2 angstrom in the hexagonal setting. The diffraction data were processed and reduced to an overall completeness of 79.0% and an R-merge of 12.0%. There are three viral capsids in the unit cell. The icosahedral threefold axis is coincident with the crystallographic threefold axis, resulting in one third of a capsid (20 monomers) per crystallographic asymmetric unit. The orientation of the viral capsid has been determined by rotation-function searches and is positioned at (0, 0, 0) by packing considerations.

引用

页码：1073 / 1076

页数：4

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