A fast brassinolide-regulated response pathway in the plasma membrane of Arabidopsis thaliana

被引:91
作者
Caesar, Katharina [1 ]
Elgass, Kirstin [1 ]
Chen, Zhonghua [2 ]
Huppenberger, Peter [1 ]
Witthoeft, Janika [1 ]
Schleifenbaum, Frank [1 ]
Blatt, Michael R. [2 ]
Oecking, Claudia [1 ]
Harter, Klaus [1 ]
机构
[1] Univ Tubingen, Dept Plant Physiol, Ctr Plant Mol Biol, D-72076 Tubingen, Germany
[2] Univ Glasgow, Lab Plant Physiol & Biophys, Fac Biomed & Life Sci, Glasgow G12 8QQ, Lanark, Scotland
基金
英国生物技术与生命科学研究理事会;
关键词
brassinolide; brassinosteroid-insensitive 1 (BRI1); fluorescence lifetime; membrane potential; P-ATPase; cell wall; ELECTROGENIC PROTON EXTRUSION; PROTEIN-PROTEIN INTERACTIONS; H+-ATPASE; INTRACELLULAR PH; FLUORESCENT PROTEINS; ABSCISIC-ACID; SENSITIVE GFP; CELL-SURFACE; PLANT-GROWTH; BRASSINOSTEROIDS;
D O I
10.1111/j.1365-313X.2011.04510.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
To understand molecular processes in living plant cells, quantitative spectro-microscopic technologies are required. By combining fluorescence lifetime spectroscopy with confocal microscopy, we studied the subcellular properties and function of a GFP-tagged variant of the plasma membrane-bound brassinosteroid receptor BRI1 (BRI1-GFP) in living cells of Arabidopsis seedlings. Shortly after adding brassinolide, we observed BRI1-dependent cell-wall expansion, preceding cell elongation. In parallel, the fluorescence lifetime of BRI1-GFP decreased, indicating an alteration in the receptor's physico-chemical environment. The parameter modulating the fluorescence lifetime of BRI1-GFP was found to be BL-induced hyperpolarization of the plasma membrane. Furthermore, for induction of hyperpolarization and cell-wall expansion, activation of the plasma membrane P-ATPase was necessary. This activation required BRI1 kinase activity, and was mediated by BL-modulated interaction of BRI1 with the P-ATPase. Our results were used to develop a model suggesting that there is a fast BL-regulated signal response pathway within the plasma membrane that links BRI1 with P-ATPase for the regulation of cell-wall expansion.
引用
收藏
页码:528 / 540
页数:13
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