A novel neuropeptide, Hym-176, induces contraction of the ectodermal muscle in Hydra

被引：58

作者：

Yum, S

Takahashi, T

Koizumi, O

Ariura, Y

Kobayakawa, Y

Mohri, S

Fujisawa, T ^{[1
]}

机构：

[1] Natl Inst Genet, Dept Dev Genet, Mishima, Shizuoka 411, Japan

[2] Fukuoka Womens Univ, Fac Human Environm Sci, Fukuoka 813, Japan

[3] Hamanoumachi Hosp, Fac Sci, Fukuoka 810, Japan

[4] Kyushu Univ, Fac Med, Fukuoka 81282, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1998年 / 248卷 / 03期

基金：

新加坡国家研究基金会;

关键词：

D O I：

10.1006/bbrc.1998.8831

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

During the course of a systematic non-targeting screening of peptide signal molecules in Hydra, we identified a novel myoactive neuropeptide called Hym-176. The primary structure of Hym-176 was determined to be APFIFPGPKV amide. It specifically and reversibly induced contraction of the ectodermal muscle of the hydra body column in vivo. However, it had no effect on the ectodermal muscle of the tentacles. The structure-activity relationship analysis showed that the sequence of FIFPGPKV amide is a minimal requirement for the myoactivity. Removal of an amide group from the C-terminus completely abolished the activity. By using the antibody specific to Hym-176, the tissue localization of the peptide in hydra was determined immunohistochemically. The intense immunoreactivity was found in the peduncle nerve cells, indicating that Hym-176 is a neuropeptide. (C) 1998 Academic Press.

引用

页码：584 / 590

页数：7

共 18 条

[1]

CAMPBELL RD, 1976, J CELL SCI, V21, P1

[2]

CAMPBELL RD, 1973, J CELL SCI, V21, P1

[3] QUANTITATIVE METHOD FOR MACERATION OF HYDRA TISSUE [J].

DAVID, CN .

WILHELM ROUX ARCHIV FUR ENTWICKLUNGSMECHANIK DER ORGANISMEN, 1973, 171 (04) :259-268

[4]

GRENS A, UNPUB

[5]

GRIMMELIKHUIJZE.CJ, 1986, HDB COMP OPIOID RELA, P103

[6]

GRIMMELIKHUIJZEN CJP, 1992, AM ZOOL, V32, P1

[7]

Leviev I, 1997, J NEUROCHEM, V68, P1319

[8] DIFFERENTIAL DISPLAY OF EUKARYOTIC MESSENGER-RNA BY MEANS OF THE POLYMERASE CHAIN-REACTION [J].

LIANG, P ;

PARDEE, AB .

SCIENCE, 1992, 257 (5072) :967-971

[9]

MCFARLANE ID, 1987, J EXP BIOL, V133, P157

[10]

MCFARLANE ID, 1991, J EXP BIOL, V156, P419

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