Heterologous expression of the Arabidopsis DREB1A/CBF3 gene enhances drought and freezing tolerance in transgenic Lolium perenne plants

被引：28

作者：

Li, Xue ^{[2
]}

Cheng, Xiaoxia ^{[2
]}

Liu, Jun ^{[2
]}

Zeng, Huiming ^{[2
]}

Han, Liebao ^{[1
,2
]}

Tang, Wei ^{[1
]}

机构：

[1] Yangtze Univ, Coll Hort & Gardening, Jinzhou 434025, Hubei Province, Peoples R China

[2] Beijing Forestry Univ, Inst Turfgrass Sci, Beijing 100084, Peoples R China

来源：

PLANT BIOTECHNOLOGY REPORTS | 2011年 / 5卷 / 01期

关键词：

Agrobacterium tumefaciens; AtDREB1A/CBF3; Drought and freezing stress; Lolium perenne; Turfgrass; TRANSCRIPTION FACTOR; LOW-TEMPERATURE; COLD-ACCLIMATION; ABIOTIC STRESS; THALIANA; RICE; DNA; IDENTIFICATION; SPECIFICITY; MICROARRAY;

D O I：

10.1007/s11816-010-0157-9

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The dehydration-responsive element binding proteins (DREB1)/C-repeat (CRT) binding factors (CBF) function as transcription factors and play an important role in agricultural biotechnology and molecular biology studies of drought and freezing stress tolerance. We generated transgenic Lolium perenne plants containing the PCR-cloned Arabidopsis DREB1A/CBF3 gene (AtDREB1A/CBF3) to study the function of this gene construct in drought and freezing tolerance in a species of turfgrass. Compared to the control, AtDREB1A/CBF3 transgenic L. perenne plants showed enhanced drought and freezing stress tolerance. The activities of the enzymes superoxide dismutase (SOD) and peroxidase (POD) were higher in transgenic plants than in the non-transgenic plant control. These results demonstrate that the expression of the AtDREB1A/CBF3 gene in transgenic L. perenne plants enhanced drought and freezing tolerance and that the increased stress tolerance was associated with the increased activities of antioxidant enzymes. These results are relevant to stress biology and biotechnology studies of turfgrass.

引用

页码：61 / 69

页数：9

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