Controlled insulin release from glucose-sensitive self-assembled multilayer films based on 21-arm star polymer

被引：122

作者：

Chen, Xingyu ^{[1
]}

Wu, Wei ^{[1
]}

Guo, Zhizhang ^{[1
]}

Xin, Jianyu ^{[1
]}

Li, Jianshu ^{[1
]}

机构：

[1] Sichuan Univ, Coll Polymer Sci & Engn, Chengdu 610065, Peoples R China

来源：

BIOMATERIALS | 2011年 / 32卷 / 06期

基金：

中国国家自然科学基金;

关键词：

Controlled release; Insulin; Multilayer films; Layer-by-layer assembly; 21-Arm star polymer; Animal experiments; THIN-FILMS; RADICAL POLYMERIZATION; POLY(ACRYLIC ACID); CYCLODEXTRIN-CORE; DELIVERY; PH; MICROCAPSULES; MORPHOLOGY; HYDROGELS; DESIGN;

D O I：

10.1016/j.biomaterials.2010.11.002

中图分类号：

R318 [生物医学工程];

学科分类号：

100103 [病原生物学];

摘要：

A glucose-sensitive multilayer film was fabricated by the layer-by-layer (LbL) assembly method with positively charged 21-arm poly[2-(dimethylamino)ethyl methacrylate] (star PDMAEMA) and negatively charged insulin and glucose oxidase (GOD) in the form of ((Star PDMAEMA/Insulin)(4) + (Star PDMAEMA/GOD)(4) + Star PDMAEMA). The multilayer film shows an on-off regulation of insulin release in response to stepwise glucose challenge in vitro. It is found that the unique structure of star PDMAEMA and inter-diffusion of charged insulin are the main factors to control the on-off status of the film. Reversible surface morphology transitions of the multilayer film were also observed, revealing a phase separation and large-scale reorganization process. Furthermore, the multilayer film could continuously release enough insulin in vivo after being subcutaneously implanted in streptocozotin-induced diabetic rats and reduce the blood glucose level for at least two weeks. It is indicated that such system may have substantial potential as a glucose-sensitive carrier for insulin due to its distinct mechanism. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：1759 / 1766

页数：8

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