Cell wall-anchored surface proteins of gram-positive pathogens play important roles during the establishment of many infectious diseases, but the contributions of surface proteins to the pathogenesis of anthrax have not yet been revealed. Cell wall anchoring in Staphylococcus aureus occurs by a transpeptidation mechanism requiring surface proteins with C-terminal sorting signals as well as sortase enzymes. The genome sequence of Bacillus anthracis encodes three sortase genes and eleven surface proteins with different types of cell wall sorting signals. Purified B. anthracis sortase A cleaved peptides encompassing LPXTG motif-type sorting signals between the threonine (T) and the glycine (G) residues in vitro. Sortase A activity could be inhibited by thiol-reactive reagents, similar to staphylococcal sortases. B. anthracis parent strain Sterne 34F(2), but not variants lacking the srt4 gene, anchored the collagen-binding MSCRAMM (microbial surface components recognizing adhesive matrix molecules) BasC (BA5258/BAS4884) to the bacterial cell wall. These results suggest that B. anthracis SrtA anchors surface proteins bearing LPXTG motif sorting signals to the cell wall envelope of vegetative bacilli.
机构:
Inst Pasteur, Unite Toxines & Pathogenie Bacteriennes, CNRS, URA 1858, F-75724 Paris 15, FranceInst Pasteur, Unite Toxines & Pathogenie Bacteriennes, CNRS, URA 1858, F-75724 Paris 15, France
Brossier, F
;
Weber-Levy, M
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Inst Pasteur, Unite Toxines & Pathogenie Bacteriennes, CNRS, URA 1858, F-75724 Paris 15, FranceInst Pasteur, Unite Toxines & Pathogenie Bacteriennes, CNRS, URA 1858, F-75724 Paris 15, France
Weber-Levy, M
;
Mock, M
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机构:
Inst Pasteur, Unite Toxines & Pathogenie Bacteriennes, CNRS, URA 1858, F-75724 Paris 15, FranceInst Pasteur, Unite Toxines & Pathogenie Bacteriennes, CNRS, URA 1858, F-75724 Paris 15, France
Mock, M
;
Sirard, JC
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机构:
Inst Pasteur, Unite Toxines & Pathogenie Bacteriennes, CNRS, URA 1858, F-75724 Paris 15, FranceInst Pasteur, Unite Toxines & Pathogenie Bacteriennes, CNRS, URA 1858, F-75724 Paris 15, France
机构:
Inst Pasteur, CNRS, URA 2172, Unite Toxines & Pathogenie Bacteriennes, F-75725 Paris 15, FranceInst Pasteur, CNRS, URA 2172, Unite Toxines & Pathogenie Bacteriennes, F-75725 Paris 15, France
Brossier, F
;
Mock, M
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h-index: 0
机构:
Inst Pasteur, CNRS, URA 2172, Unite Toxines & Pathogenie Bacteriennes, F-75725 Paris 15, FranceInst Pasteur, CNRS, URA 2172, Unite Toxines & Pathogenie Bacteriennes, F-75725 Paris 15, France
机构:
INST PASTEUR, UNITE ANTIGENES BACTERIENS, 28 RUE DR ROUX, F-75724 PARIS 15, FRANCEINST PASTEUR, UNITE ANTIGENES BACTERIENS, 28 RUE DR ROUX, F-75724 PARIS 15, FRANCE
CATALDI, A
;
LABRUYERE, E
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INST PASTEUR, UNITE ANTIGENES BACTERIENS, 28 RUE DR ROUX, F-75724 PARIS 15, FRANCEINST PASTEUR, UNITE ANTIGENES BACTERIENS, 28 RUE DR ROUX, F-75724 PARIS 15, FRANCE
LABRUYERE, E
;
MOCK, M
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h-index: 0
机构:
INST PASTEUR, UNITE ANTIGENES BACTERIENS, 28 RUE DR ROUX, F-75724 PARIS 15, FRANCEINST PASTEUR, UNITE ANTIGENES BACTERIENS, 28 RUE DR ROUX, F-75724 PARIS 15, FRANCE
机构:
Inst Pasteur, Unite Toxines & Pathogenie Bacteriennes, CNRS, URA 1858, F-75724 Paris 15, FranceInst Pasteur, Unite Toxines & Pathogenie Bacteriennes, CNRS, URA 1858, F-75724 Paris 15, France
Brossier, F
;
Weber-Levy, M
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h-index: 0
机构:
Inst Pasteur, Unite Toxines & Pathogenie Bacteriennes, CNRS, URA 1858, F-75724 Paris 15, FranceInst Pasteur, Unite Toxines & Pathogenie Bacteriennes, CNRS, URA 1858, F-75724 Paris 15, France
Weber-Levy, M
;
Mock, M
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h-index: 0
机构:
Inst Pasteur, Unite Toxines & Pathogenie Bacteriennes, CNRS, URA 1858, F-75724 Paris 15, FranceInst Pasteur, Unite Toxines & Pathogenie Bacteriennes, CNRS, URA 1858, F-75724 Paris 15, France
Mock, M
;
Sirard, JC
论文数: 0引用数: 0
h-index: 0
机构:
Inst Pasteur, Unite Toxines & Pathogenie Bacteriennes, CNRS, URA 1858, F-75724 Paris 15, FranceInst Pasteur, Unite Toxines & Pathogenie Bacteriennes, CNRS, URA 1858, F-75724 Paris 15, France
机构:
Inst Pasteur, CNRS, URA 2172, Unite Toxines & Pathogenie Bacteriennes, F-75725 Paris 15, FranceInst Pasteur, CNRS, URA 2172, Unite Toxines & Pathogenie Bacteriennes, F-75725 Paris 15, France
Brossier, F
;
Mock, M
论文数: 0引用数: 0
h-index: 0
机构:
Inst Pasteur, CNRS, URA 2172, Unite Toxines & Pathogenie Bacteriennes, F-75725 Paris 15, FranceInst Pasteur, CNRS, URA 2172, Unite Toxines & Pathogenie Bacteriennes, F-75725 Paris 15, France
机构:
INST PASTEUR, UNITE ANTIGENES BACTERIENS, 28 RUE DR ROUX, F-75724 PARIS 15, FRANCEINST PASTEUR, UNITE ANTIGENES BACTERIENS, 28 RUE DR ROUX, F-75724 PARIS 15, FRANCE
CATALDI, A
;
LABRUYERE, E
论文数: 0引用数: 0
h-index: 0
机构:
INST PASTEUR, UNITE ANTIGENES BACTERIENS, 28 RUE DR ROUX, F-75724 PARIS 15, FRANCEINST PASTEUR, UNITE ANTIGENES BACTERIENS, 28 RUE DR ROUX, F-75724 PARIS 15, FRANCE
LABRUYERE, E
;
MOCK, M
论文数: 0引用数: 0
h-index: 0
机构:
INST PASTEUR, UNITE ANTIGENES BACTERIENS, 28 RUE DR ROUX, F-75724 PARIS 15, FRANCEINST PASTEUR, UNITE ANTIGENES BACTERIENS, 28 RUE DR ROUX, F-75724 PARIS 15, FRANCE