Preparation of chitosan particles suitable for enzyme immobilization

被引：137

作者：

Biro, Emese ^{[1
]}

Nemeth, Agnes Sz. ^{[1
]}

Sisak, Csaba ^{[1
]}

Feczko, Tivadar ^{[1
,2
]}

Gyenis, Janos ^{[1
]}

机构：

[1] Univ Pannonia, Fac Informat Technol, Res Inst Chem & Proc Engn, H-8200 Veszprem, Hungary

[2] Hungarian Acad Sci, Chem Res Ctr, Inst Mat & Environm Chem, H-1025 Budapest, Hungary

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 2008年 / 70卷 / 06期

关键词：

chitosan; microspheres; nanoparticles; beta-galactosidase; immobilization;

D O I：

10.1016/j.jprot.2007.11.005

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Macro-, micro- and nanosized chitosan particles suitable as immobilization carriers were prepared by precipitation, emulsion cross-linking and ionic gelation methods, respectively. Effects of particle preparation parameters on particle size were investigated. Activities of beta-galactosidase covalently attached to differently sized particles have been evaluated and compared. The highest activity was shown by the biocatalyst immobilized on nanoparticles obtained by means of the ionotropic gelation method with sodium sulphate as gelation agent. beta-Galactosidase fixed on macro- and microspheres exhibited excellent storage stability in aqueous solution, with no more than 5% loss of activity after 3 weeks storage at 4 degrees C and pH 7.0. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：1240 / 1246

页数：7

共 27 条

[1] Recent advances on chitosan-based micro- and nanoparticles in drug delivery
Agnihotri, SA
Mallikarjuna, NN
Aminabhavi, TM
[J]. JOURNAL OF CONTROLLED RELEASE, 2004, 100 (01) : 5 - 28
[2] Preparation and characterization of chitosan microspheres as drug carrier for prednisolone sodium phosphate as model for antiinflammatory drugs
Berthold, A
Cremer, K
Kreuter, J
[J]. JOURNAL OF CONTROLLED RELEASE, 1996, 39 (01) : 17 - 25
[3] BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[4] β-galactosidase from Penicillium canescens.: Properties and immobilization
Budriene, S
Gorochovceva, N
Romaskevic, T
Yugova, LV
Miezeliene, A
Dienys, G
Zubriene, A
[J]. CENTRAL EUROPEAN JOURNAL OF CHEMISTRY, 2005, 3 (01): : 95 - 105
[5] Determination of kinetics parameters for free and immobilized beta-galactosidase
Carrara, CR
Rubiolo, AC
[J]. PROCESS BIOCHEMISTRY, 1996, 31 (03) : 243 - 248
[6] CAVAILLE D, 1995, BIOTECHNOL APPL BIOC, V22, P55
[7] Preparation and biocompatibility of chitosan microcarriers as biomaterial
Chen, XG
Liu, CS
Liu, CG
Meng, XH
Lee, CM
Park, HJ
[J]. BIOCHEMICAL ENGINEERING JOURNAL, 2006, 27 (03) : 269 - 274
[8] CHIBATA I, 1978, IMMOBILIZED ENZYMES, P2
[9] Immobilization of β-cyclodextrin in chitosan beads for separation of cholesterol from egg yolk
Chiu, SH
Chung, TW
Giridhar, R
Wu, WT
[J]. FOOD RESEARCH INTERNATIONAL, 2004, 37 (03) : 217 - 223
[10] Denkbas EB, 2000, J APPL POLYM SCI, V76, P1637, DOI 10.1002/(SICI)1097-4628(20000613)76:11<1637::AID-APP4>3.0.CO

← 1 2 3 →