In situ visualization of DSBs to assess the extranuclear/extracellular effects induced by low-dose α-particle irradiation

被引:43
作者
Hu, B
Han, W
Wu, LJ
Feng, HY
Liu, XL
Zhang, LL
Xu, A
Hei, TK
Yu, ZL
机构
[1] Chinese Acad Sci, Inst Plasma Phys, Key Lab Ion Beam Bioengn, Hefei 230031, Peoples R China
[2] Columbia Univ Coll Phys & Surg, Ctr Radiol Res, New York, NY 10032 USA
关键词
D O I
10.1667/RR3415.1
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Extranuclear/extracellular effects may have a significant effect on low-dose radiation risk assessment as well as on the shape of the dose-response relationship. Numerous studies using different end points such as sister chromatid exchanges, micronuclei and mutation have shown that this phenomenon exists in many cell types. However, these end points mostly reflect the late events after radiation damage, and little is known about the early response in this phenomenon. DNA double-strand breaks (DSBs) induced by ionizing radiation or carcinogenic chemicals can be visualized in situ using gamma-H2AX immunofluorescence staining, and there is evidence that the number of gamma-H2AX foci can be closely correlated with DSBs induced. Here we used gamma-H2AX as a biomarker to assess the extranuclear/extracellular effects induced by low-dose alpha particles in situ. The results show that a greater fraction of positive cells with DSBs (48.6%) was observed than the number of cells whose nuclei were actually traversed by the 1-cGy dose of (x particles (9.2%). The fraction of DSB-positive cells was greatly reduced after treatment with either lindane or DMSO. These results suggest that in situ visualization of DSBs can be used to assess radiation-induced extranuclear/extracellular effects soon after irradiation. Moreover, the in situ DSB assay may provide a means to evaluate the spatial effect on unirradiated cells that are located in the neighboring region of cells irradiated by alpha particles. (c) 2005 by Radiation Research Society.
引用
收藏
页码:286 / 291
页数:6
相关论文
共 42 条
[1]   Dynamics of DNA double-strand breaks revealed by clustering of damaged chromosome domains [J].
Aten, JA ;
Stap, J ;
Krawczyk, PM ;
van Oven, CH ;
Hoebe, RA ;
Essers, J ;
Kanaar, R .
SCIENCE, 2004, 303 (5654) :92-95
[2]   Direct evidence for the participation of gap junction-mediated intercellular communication in the transmission of damage signals from α-particle irradiated to nonirradiated cells [J].
Azzam, EI ;
de Toledo, SM ;
Little, JB .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (02) :473-478
[3]   The switch from survival responses to apoptosis after chromosomal breaks [J].
Bree, RT ;
Neary, C ;
Samali, A ;
Lowndes, NF .
DNA REPAIR, 2004, 3 (8-9) :989-995
[4]  
Burma S, 2001, J BIOL CHEM, V276, P42462, DOI 10.1074/jbc.C100466200
[5]   Reactivity of human apurinic/apyrimidinic endonuclease and Escherichia coli exonuclease III with bistranded abasic sites in DNA [J].
Chaudhry, MA ;
Weinfeld, M .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (25) :15650-15655
[6]   Response to RAG-mediated V(D)J cleavage by NBS1 and γ-H2AX [J].
Chen, HT ;
Bhandoola, A ;
Difilippantonio, MJ ;
Zhu, J ;
Brown, MJ ;
Tai, XG ;
Rogakou, EP ;
Brotz, TM ;
Bonner, WM ;
Ried, T ;
Nussenzweig, A .
SCIENCE, 2000, 290 (5498) :1962-1964
[7]  
d'Adda diFagagna F., 2003, Nature, V426, P194, DOI DOI 10.1038/NATURE02118
[8]  
Dahm-Daphi J, 2000, INT J RADIAT BIOL, V76, P67, DOI 10.1080/095530000139023
[9]   High NaCl causes Mre11 to leave the nucleus, disrupting DNA damage signaling and repair [J].
Dmitrieva, NI ;
Bulavin, DV ;
Burg, MB .
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY, 2003, 285 (02) :F266-F274
[10]   The in vitro micronucleus technique [J].
Fenech, M .
MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, 2000, 455 (1-2) :81-95