rpoB mutations in multidrug-resistant strains of Mycobacterium tuberculosis isolated in Italy

被引：78

作者：

Pozzi, G

Meloni, M

Iona, E

Orrù, G

Thoresen, OF

Ricci, ML

Oggioni, MR

Fattorini, L

Orefici, G

机构：

[1] Univ Siena, Dipartimento Biol Mol, Sez Microbiol, I-53100 Siena, Italy

[2] Univ Cagliari, Dipartimento Sci Chirurg, Cagliari, Italy

[3] Ist Super Sanita, Batteriol & Micol Med Lab, I-00161 Rome, Italy

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1999年 / 37卷 / 04期

关键词：

D O I：

10.1128/JCM.37.4.1197-1199.1999

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Mutations of rpoB associated with rifampin resistance were studied in 37 multidrug-resistant (MDR) clinical strains of Mycobacterium tuberculosis isolated in Italy. At least lone mutated codon was found in each MDR strain. It was always a single-base substitution leading to an amino acid change. Nine different rpoB alleles, three of which had not been reported before, were found. The relative frequencies of specific mutations in this sample were different from those previously reported from different geographical areas, since 22 strains (59.5%) carried the mutated codon TTG in position 531 (Ser-->Leu) and 11 (29.7%) had GAC in position 526 (His-->Asp).

引用

收藏

页码：1197 / 1199

页数：3

相关论文

共 19 条

[1] TREATMENT OF TUBERCULOSIS AND TUBERCULOSIS INFECTION IN ADULTS AND CHILDREN [J].

BASS, JB ;

FARER, LS ;

HOPEWELL, PC ;

OBRIEN, R ;

JACOBS, RF ;

RUBEN, F ;

SNIDER, DE ;

THORNTON, G .

AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 1994, 149 (05) :1359-1374

[2] Detection of rifampin resistance among isolates of Mycobacterium tuberculosis from Mozambique [J].

Caugant, DA ;

Sandven, P ;

Eng, J ;

Jeque, JT ;

Tonjum, T .

MICROBIAL DRUG RESISTANCE-MECHANISMS EPIDEMIOLOGY AND DISEASE, 1995, 1 (04) :321-326

[3] Evaluation of a line probe assay kit for characterization of rpoB mutations in rifampin-resistant Mycobacterium tuberculosis isolates from new York City [J].

Cooksey, RC ;

Morlock, GP ;

Glickman, S ;

Crawford, JT .

JOURNAL OF CLINICAL MICROBIOLOGY, 1997, 35 (05) :1281-1283

[4]

HEIFETS LB, 1991, DRUG SUSCEPTIBILITY, P14

[5] IMPLICATIONS OF MULTIDRUG-RESISTANCE FOR THE FUTURE OF SHORT-COURSE CHEMOTHERAPY OF TUBERCULOSIS - A MOLECULAR STUDY [J].

HEYM, B ;

HONORE, N ;

TRUFFOTPERNOT, C ;

BANERJEE, A ;

SCHURRA, C ;

JACOBS, WR ;

VANEMBDEN, JD ;

GROSSET, JH ;

COLE, ST .

LANCET, 1994, 344 (8918) :293-298

[6] CHARACTERIZATION BY AUTOMATED DNA-SEQUENCING OF MUTATIONS IN THE GENE (RPOB) ENCODING THE RNA-POLYMERASE BETA-SUBUNIT IN RIFAMPIN-RESISTANT MYCOBACTERIUM-TUBERCULOSIS STRAINS FROM NEW-YORK-CITY AND TEXAS [J].

KAPUR, V ;

LI, LL ;

IORDANESCU, S ;

HAMRICK, MR ;

WANGER, A ;

KREISWIRTH, BN ;

MUSSER, JM .

JOURNAL OF CLINICAL MICROBIOLOGY, 1994, 32 (04) :1095-1098

[7] Characterization of rpoB mutations in rifampin-resistant clinical Mycobacterium tuberculosis isolates from Greece [J].

Matsiota-Bernard, P ;

Vrioni, G ;

Marinis, E .

JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (01) :20-23

[8] Comparative antimycobacterial activities of rifampin, rifapentine, and KRM-1648 against a collection of rifampin-resistant Mycobacterium tuberculosis isolates with known rpoB mutations [J].

Moghazeh, SL ;

Pan, X ;

Arain, T ;

Stover, CK ;

Musser, JM ;

Kreiswirth, BN .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1996, 40 (11) :2655-2657

[9] MOLECULAR MECHANISMS OF MULTIPLE-DRUG RESISTANCE IN CLINICAL ISOLATES OF MYCOBACTERIUM-TUBERCULOSIS [J].

MORRIS, S ;

BAI, GH ;

SUFFYS, P ;

PORTILLOGOMEZ, L ;

FAIRCHOK, M ;

ROUSE, D .

JOURNAL OF INFECTIOUS DISEASES, 1995, 171 (04) :954-960

[10] ANTIMICROBIAL AGENT RESISTANCE IN MYCOBACTERIA - MOLECULAR-GENETIC INSIGHTS [J].

MUSSER, JM .

CLINICAL MICROBIOLOGY REVIEWS, 1995, 8 (04) :496-&