Direct detection of Campylobacter jejuni in chicken cecal contents by PCR

被引：21

作者：

Chuma, T

Yano, K

Omori, H

Okamoto, K

Yugi, H

机构：

[1] Dept. of Veterinary Public Health, Faculty of Agriculture, Kagoshima University

来源：

JOURNAL OF VETERINARY MEDICAL SCIENCE | 1997年 / 59卷 / 01期

关键词：

Campylobacter; chicken; PCR;

D O I：

10.1292/jvms.59.85

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Campylobacter jejuni in chicken feces was detected by PCR and Southern blot hybridization (SBH). The detection limits of C. jejuni in chicken feces were 34,000 cells by PCR and 340 cells by SBH. Some cecal contents of chickens up to 3 weeks old were C. jejuni positive by SBH whereas all of them were negative by PCR. Two of 51 cecal contents of 18-day-old chicken embryos were C. jejuni positive by PCR and SBH; but, C. jejuni were not isolated from the samples by conventional culture with selective enrichment.

引用

收藏

页码：85 / 87

页数：3

相关论文

共 25 条

[1] THE MODE OF SPREAD OF CAMPYLOBACTER-JEJUNI-COLI TO BROILER FLOCKS [J].

ANNANPRAH, A ;

JANC, M .

JOURNAL OF VETERINARY MEDICINE SERIES B-INFECTIOUS DISEASES AND VETERINARY PUBLIC HEALTH, 1988, 35 (01) :11-18

[2] CHICKEN-TO-HUMAN INFECTION WITH CAMPYLOBACTER-JEJUNI AND CAMPYLOBACTER-COLI - BIOTYPE AND SEROTYPE CORRELATION [J].

ANNANPRAH, A ;

JANC, M .

JOURNAL OF FOOD PROTECTION, 1988, 51 (07) :562-564

[3] RESERVOIRS FOR HUMAN CAMPYLOBACTERIOSIS [J].

BLASER, MJ ;

LAFORCE, FM ;

WILSON, NA ;

WANG, WLL .

JOURNAL OF INFECTIOUS DISEASES, 1980, 141 (05) :665-669

[4] APPLICATION OF A DNA-DNA HYBRIDIZATION METHOD FOR DETECTION OF CAMPYLOBACTER-JEJUNI IN CHICKEN FECES [J].

CHUMA, T ;

YAMADA, T ;

OKAMOTO, K ;

YUGI, H ;

OHYA, T .

JOURNAL OF VETERINARY MEDICAL SCIENCE, 1993, 55 (06) :1027-1029

[5] A SURVEY OF CAMPYLOBACTER-JEJUNI IN BROILERS FROM ASSIGNMENT TO SLAUGHTER USING DNA-DNA HYBRIDIZATION [J].

CHUMA, T ;

YAMADA, T ;

YANO, K ;

OKAMOTO, K ;

YUGI, H .

JOURNAL OF VETERINARY MEDICAL SCIENCE, 1994, 56 (04) :697-700

[6]

DOYLE MP, 1984, APPL ENVIRON MICROB, V47, P533, DOI 10.1128/AEM.47.3.533-536.1984

[7] RAPID AND SENSITIVE DETECTION OF CAMPYLOBACTER SPP IN CHICKEN PRODUCTS BY USING THE POLYMERASE CHAIN-REACTION [J].

GIESENDORF, BAJ ;

QUINT, WGV ;

HENKENS, MHC ;

STEGEMAN, H ;

HUF, FA ;

NIESTERS, HGM .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1992, 58 (12) :3804-3808

[8] BROILER-CHICKENS AS POTENTIAL SOURCE OF CAMPYLOBACTER INFECTIONS IN HUMANS [J].

GRANT, IH ;

RICHARDSON, NJ ;

BOKKENHEUSER, VD .

JOURNAL OF CLINICAL MICROBIOLOGY, 1980, 11 (05) :508-510

[9] DETECTION OF THE COCCOID FORM OF CAMPYLOBACTER-JEJUNI IN CHICKEN PRODUCTS WITH THE USE OF THE POLYMERASE CHAIN-REACTION [J].

HAZELEGER, W ;

ARKESTEIJN, C ;

TOOROPBOUMA, A ;

BEUMER, R .

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 1994, 24 (1-2) :273-281

[10] THE EXTENT OF SURFACE CONTAMINATION OF RETAILED CHICKENS WITH CAMPYLOBACTER-JEJUNI SEROGROUPS [J].

HOOD, AM ;

PEARSON, AD ;

SHAHAMAT, M .

EPIDEMIOLOGY AND INFECTION, 1988, 100 (01) :17-25