Increase in fecal primary bile acids and dysbiosis in patients with diarrhea-predominant irritable bowel syndrome

被引:207
作者
Duboc, H. [1 ,2 ,3 ]
Rainteau, D. [3 ]
Rajca, S. [3 ]
Humbert, L. [3 ]
Farabos, D. [3 ]
Maubert, M. [3 ]
Grondin, V. [3 ]
Jouet, P. [1 ,2 ]
Bouhassira, D. [2 ]
Seksik, P. [3 ]
Sokol, H. [3 ]
Coffin, B. [1 ,2 ]
Sabate, J. M. [1 ,2 ]
机构
[1] Univ Paris 07, Louis Mourier Hosp, AP HP, Hepato Gastro Enterol Dept, Colombes, France
[2] Ambroise Pare Hosp, INSERM, U987, Pain Pathophysiol & Pharmacol Unit, Boulogne, France
[3] Univ Paris 06, INSERM, U1057, ERL,UMR 7203, Paris, France
关键词
7; alpha-dehydroxylation; bile acids; diarrhea; dysbiosis; IBS; microbiota; COLONIC EPITHELIAL-CELLS; INDUCED SECRETION; RABBIT COLON; MICROBIOTA; MALABSORPTION; EPIDEMIOLOGY; PERMEABILITY; CONSTIPATION; MONOLAYERS; DIAGNOSIS;
D O I
10.1111/j.1365-2982.2012.01893.x
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Background Irritable bowel syndrome (IBS) is a multifactorial disease for which a dysbiosis of the gut microbiota has been described. Bile acids (BA) could play a role as they are endogenous laxatives and are metabolized by gut microbiota. We compared fecal BA profiles and microbiota in healthy subjects (HS) and patients with diarrhea-predominant IBS (IBS-D), and we searched for an association with symptoms. Methods Clinical features and stool samples were collected in IBS-D patients and HS. Fecal BA profiles were generated using HPLC coupled to tandem mass spectrometry. The fecal microbiota composition was assessed by q-PCR targeting dominant bacterial groups and species implicated in BA transformation. Key Results Fourteen IBS-D patients and 18 HS were included. The two groups were comparable in terms of age and sex. The percentage of fecal primary BA was significantly higher in IBS-D patients than in HS, and it was significantly correlated with stool consistency and frequency. Fecal counts of all bacteria, lactobacillus, coccoides, leptum and Faecalibacterium prausnitzii were similar. There was a significant increase of Escherichia coli and a significant decrease of leptum and bifidobacterium in IBS-D patients. Conclusions & Inferences We report an increase of primary BA in the feces of IBS-D patients compared to HS, correlated with stool consistency and frequency. A dysbiosis of different bacterial groups was detected, some of them involved in BA transformation. As the gut microbiota is the exclusive pathway to transform primary into secondary BA, this suggests a functional consequence of dysbiosis, leading to lower BA transformation.
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页码:513 / +
页数:10
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