Cloning and characterization of AWP1, a novel protein that associates with serine/threonine kinase PRK1 in vivo

被引：39

作者：

Duan, W ^{[1
]}

Sun, BG ^{[1
]}

Li, TW ^{[1
]}

Tan, BJ ^{[1
]}

Lee, MK ^{[1
]}

Teo, TS ^{[1
]}

机构：

[1] Natl Univ Singapore, Fac Med, Dept Biochem, Singapore 119260, Singapore

来源：

GENE | 2000年 / 256卷 / 1-2期

关键词：

PKN; protein-protein interaction; signal transduction;

D O I：

10.1016/S0378-1119(00)00365-6

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We describe the cloning and expression of cDNAs encoding a novel human protein of 208 amino acid residues with a predicted molecular mass of 22.6 kDa and its mouse homologue. We name this protein as AWP1 (associated with PRK1). AWP1 is a ubiquitously expressed protein, and the Awp1 gene is switched on during early human and mouse development. When expressed in COS-I cells, the Myc-tagged AWP1 has an apparent molecular mass higher than that deduced from its amino acid sequence. AWP1 possesses a conserved zf-A20 zinc finger domain at its N-terminal and a zf-AN1 zinc finger domain at its C-terminal. Co-immunoprecipitation experiments revealed that mouse AWP1 specifically interacts with a rat serine/threonine protein kinase PRK1 in vivo. Hence, AWP1 may play a regulatory role in mammalian signal transduction pathways. (C) 2000 Published by Elsevier Science B.V. All rights reserved.

引用

页码：113 / 121

页数：9

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