Selective liquid CO2 extraction of purine alkaloids in different Ilex paraguariensis progenies grown under environmental influences

被引:23
作者
Cardozo, Euclides Lara, Jr.
Cardozo-Filho, Lucio
Filho, Osvaldo Ferrarese
Zanoelo, Everton Fernando
机构
[1] Univ Paranaense, Dept Pharm, BR-85903170 Toledo, Parana, Brazil
[2] Univ Estadual Maringa, Dept Chem Engn, BR-87020900 Maringa, Parana, Brazil
[3] Univ Estadual Maringa, Dept Biochem, BR-87020900 Maringa, Parana, Brazil
[4] Univ Fed Parana, Dept Chem Engn, Polytech Ctr, BR-81530900 Curitiba, Parana, Brazil
关键词
compressed; carbon dioxide; extraction; progeny; Ilex paraguariensis; mate; SUPERCRITICAL CARBON-DIOXIDE; MATE ILEX; FLUID EXTRACTION; GREEN TEA; CAFFEINE; LEAVES; THEOBROMINE; METHYLXANTHINES; SOLUBILITIES; INHIBITORS;
D O I
10.1021/jf0706225
中图分类号
S [农业科学];
学科分类号
082806 [农业信息与电气工程];
摘要
In this investigation, liquid carbon dioxide at 20 degrees C and 150 bar was used for the selective extraction of caffeine and theobromine from dry leaves of mate. A comparison between the chromatograms from CO2 extraction and traditional solvent extraction supports the selectivity of carbon dioxide for these purine alkaloids. The advantages of selective liquid CO2 extraction in terms of speed and resolution of UV/HPLC is also evidenced. A randomized block design of experiments was proposed to investigate the influence of 16 progenies of Ilex paraguariensis grown in 3 diverse sites on the contents of caffeine and theobromine in liquors of mate leaves obtained by extraction with compressed CO2. A significant effect of both these factors on the parameters investigated was observed by involving the F distribution in the statistical analysis. A cluster analysis based on the experimental uncertainties in the contents of these two methylxanthines has identified from four to six different groups of mate progenies.
引用
收藏
页码:6835 / 6841
页数:7
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