A five-nucleotide sequence protects DNA from exonucleolytic degradation by AddAB, the RecBCD analogue of Bacillus subtilis

被引:66
作者
Chédin, F [1 ]
Noirot, P [1 ]
Biaudet, V [1 ]
Ehrlich, SD [1 ]
机构
[1] INRA, Lab Genet Microbienne, F-78352 Jouy En Josas, France
关键词
D O I
10.1046/j.1365-2958.1998.01018.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Homologous recombination in Bacillus subtilis requires the product of the addA and addB genes, the AddAB enzyme, This enzyme, which is both a helicase and a powerful nuclease, is thought to be the counterpart of the Escherichia coli RecBCD enzyme, From this analogy, it is expected that the nuclease activity of AddAB can be downregulated by a specific DNA sequence, which would correspond to the chi site in E. coli, Using protection of linear double-stranded DNA as a criterion, we identified the five-nucleotide sequence 5'-AGCGG-3', or its complement B'-CCGCT-3', as being sufficient for AddAB nuclease attenuation, We have shown further that this attenuation occurs only if the sequence is properly oriented with respect to the translocating AddAB enzyme, Finally, inspection of the complete B. subtilis genome revealed that this five-nucleotide sequence is over-represented and is, in a majority of cases, co-oriented with DNA replication. Based on these observations, we propose that 5'-AGCGG-3', or its complement, is the B. subtilis analogue of the E. coli chi sequence.
引用
收藏
页码:1369 / 1377
页数:9
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[1]   CHARACTERIZATION OF BACILLUS-SUBTILIS RECOMBINATIONAL PATHWAYS [J].
ALONSO, JC ;
LUDER, G ;
TAILOR, RH .
JOURNAL OF BACTERIOLOGY, 1991, 173 (13) :3977-3980
[2]   GENETIC-RECOMBINATION IN BACILLUS-SUBTILIS 168 - EFFECT OF RECN, RECF, RECH AND ADDAB MUTATIONS ON DNA-REPAIR AND RECOMBINATION [J].
ALONSO, JC ;
STIEGE, AC ;
LUDER, G .
MOLECULAR & GENERAL GENETICS, 1993, 239 (1-2) :129-136
[3]   The translocating RecBCD enzyme stimulates recombination by directing RecA protein onto ssDNA in a chi-regulated manner [J].
Anderson, DG ;
Kowalczykowski, SC .
CELL, 1997, 90 (01) :77-86
[4]   The recombination hot spot chi is a regulatory element that switches the polarity of DNA degradation by the RecBCD enzyme [J].
Anderson, DG ;
Kowalczykowski, SC .
GENES & DEVELOPMENT, 1997, 11 (05) :571-581
[5]   The recombination hotspot Chi is recognized by the translocating RecBCD enzyme as the single strand of DNA containing the sequence 5'-GCTGGTGG-3' [J].
Bianco, PR ;
Kowalczykowski, SC .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (13) :6706-6711
[6]   Computerized genetic map of Bacillus subtilis [J].
Biaudet, V ;
Samson, F ;
Anagnostopoulos, C ;
Ehrlich, SD ;
Bessieres, P .
MICROBIOLOGY-SGM, 1996, 142 :2669-2729
[7]   A 7-BASE-PAIR SEQUENCE PROTECTS DNA FROM EXONUCLEOLYTIC DEGRADATION IN LACTOCOCCUS-LACTIS [J].
BISWAS, I ;
MAGUIN, E ;
EHRLICH, SD ;
GRUSS, A .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (06) :2244-2248
[8]   PRIMOSOME ASSEMBLY SITE IN BACILLUS-SUBTILIS [J].
BRUAND, C ;
EHRLICH, SD ;
JANNIERE, L .
EMBO JOURNAL, 1995, 14 (11) :2642-2650
[9]   DNA-SEQUENCE AND ANALYSIS OF 136 KILOBASES OF THE ESCHERICHIA-COLI GENOME - ORGANIZATIONAL SYMMETRY AROUND THE ORIGIN OF REPLICATION [J].
BURLAND, V ;
PLUNKETT, G ;
DANIELS, DL ;
BLATTNER, FR .
GENOMICS, 1993, 16 (03) :551-561
[10]   RELATIONSHIP BETWEEN MOLECULAR-STRUCTURE AND TRANSFORMATION EFFICIENCY OF SOME S AUREUS PLASMIDS ISOLATED FROM B-SUBTILIS [J].
CANOSI, U ;
MORELLI, G ;
TRAUTNER, TA .
MOLECULAR & GENERAL GENETICS, 1978, 166 (03) :259-267