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Tissue plasminogen activator inhibits P-glycoprotein activity in brain endothelial cells

被引：11
作者：
Deli, MA
Abrahám, CS
Takahata, H
Niwa, M
机构：
[1] Nagasaki Univ, Sch Med, Dept Pharmacol 1, Nagasaki 8528523, Japan
[2] Nagasaki Univ, Sch Med, Dept Neurosurg, Nagasaki 8528523, Japan
[3] Hungarian Acad Sci, Biol Res Ctr, Inst Biophys, Mol Neurobiol Lab, H-6701 Szeged, Hungary
来源：
EUROPEAN JOURNAL OF PHARMACOLOGY | 2001年 / 411卷 / 1-2期
基金：
日本学术振兴会;
关键词：
blood-brain barrier; P-glycoprotein; tissue plasminogen activator;
D O I：
10.1016/S0014-2999(00)00895-5
中图分类号：
R9 [药学];
学科分类号：
1007 [药学];
摘要：
Tissue plasminogen activator (0.01-30 mug/ml) dose-dependently inhibited the functional activity of P-glycoprotein, assessed by rhodamine 123 accumulation in GP8 immortalized rat brain endothelial cells, but this effect was unrelated to its proteolytic activity. Elevation of intra-endothelial cyclic AMP concentration and stimulation of protein kinase C increased P-glycoprotein activity in GP8 cells and also attenuated the tissue plasminogen activator-induced inhibition. (C) 2001 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：R3 / R5
页数：3
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