Genetic analysis in fungi using restriction-enzyme-mediated integration

被引：41

作者：

Riggle, PJ ^{[1
]}

Kumamoto, CA ^{[1
]}

机构：

[1] Tufts Univ, Sch Med, Dept Mol Biol & Microbiol, Boston, MA 02111 USA

来源：

CURRENT OPINION IN MICROBIOLOGY | 1998年 / 1卷 / 04期

关键词：

D O I：

10.1016/S1369-5274(98)80055-6

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Restriction-enzyme-mediated integration (REMI), a method for generating nonhomologous integration of transforming DNA into the chromosomes of eukaryotic cells, has been used for insertion mutagenesis and other genetic studies in diverse organisms. Insertion mutations generated by REMI have facilitated the genetic dissection of developmental pathways in Dictyostelium discoidium and the isolation of virulence factors in several plant pathogenic fungi. Recent work indicates that REMI occurs by nonhomologous end joining.

引用

页码：395 / 399

页数：5

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