Microbial hydroxylation of 13β-ethyl-4-gonene-3,17-dione

被引：11

作者：

Jekkel, A ^{[1
]}

Ilkoy, E ^{[1
]}

Horvath, G ^{[1
]}

Pallagi, I ^{[1
]}

Suto, J ^{[1
]}

Ambrus, G ^{[1
]}

机构：

[1] Inst Drug Res, H-1045 Budapest, Hungary

来源：

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC | 1998年 / 5卷 / 1-4期

关键词：

steroid hydroxylation; fusarium nivale; 15; alpha-hydroxylation;

D O I：

10.1016/S1381-1177(98)00097-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Among the microbiological transformations of steroids 15 alpha-hydroxylation of 13 beta-ethyl-4-gonene-3,17-dione is an industrially important one [Von H. Hofmeister, K. Annen, H. Laurent, K. Petzoldt, R. Wiechert, Arzneim.-Forsch. 36 (1986) 781], since it results in an intermediate of the synthesis of Gestoden, a widely used contraceptive drug. The aim of our research was to select fungal strains for hydroxylation of 13 beta-ethyl-4-gonene-3,17-dione which produce the 15 alpha-hydroxylated product in a high yield. According to our taxonomical studies, several species of Aspergillus, Fusarium, Mortierella, and Penicillium genera fulfill this requirement. It has been reported that the 15 alpha-hydroxylating enzyme of Penicillium raistrickii is inducible by various steroidal compounds [S. Irrgang, D. Schlosser, H.-P. Schmauder, Biotechnol. Lett. 14 (1992) 33]. We found that the enzyme of Fusarium nivale (VJ-63 strain) is also advantageously induced by norethisterone, which significantly increased the economic efficiency of this biotransformation process [A. Jekkel, E. Ilkoy, J. Suto, G. Ambrus, Gy. Horvath, I. Bosinger, I. Pallagi, I. Lang, E. Gyepessy, Hungarian Patent Appl. P-9602249 (1996)]. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：385 / 387

页数：3