Manipulation of herpes simplex virus type 1 by dielectrophoresis

被引：136

作者：

Hughes, MP

Morgan, H ^{[1
]}

Rixon, FJ

Burt, JPH

Pethig, R

机构：

[1] Univ Glasgow, Dept Elect & Elect Engn, Bioelect Res Ctr, Glasgow G12 8QQ, Lanark, Scotland

[2] Univ Glasgow, MRC, Virol Unit, Glasgow G12 8QQ, Lanark, Scotland

[3] Univ Wales, Inst Mol & Biomol Elect, Bangor LL57 1UT, Gwynedd, Wales

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS | 1998年 / 1425卷 / 01期

基金：

英国生物技术与生命科学研究理事会;

关键词：

AC electrokinetics; dielectric property; nanotechnology; particle manipulation;

D O I：

10.1016/S0304-4165(98)00058-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The frequency-dependent dielectrophoretic behaviour of an enveloped mammalian virus, herpes simplex virus type 1 is described. It is demonstrated that over the range 10 kHz-20 MHz, these viral particles, when suspended in an aqueous medium of conductivity 5 mS m(-1), can be manipulated by both positive and negative dielectrophoresis using microfabricated electrode arrays. The observed transition from positive to negative dielectrophoresis at frequencies around 4.5 MHz is in qualitative agreement with a simple model of the virus as a conducting particle surrounded by an insulating membrane. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：119 / 126

页数：8

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