Isolation of human periosteum-derived progenitor cells using immunophenotypes for chondrogenesis

被引：40

作者：

Lim, SM

Choi, YS

Shin, HC

Lee, CW

Kim, DI ^{[1
]}

机构：

[1] Inha Univ, Dept Biol Engn, Inchon 402751, South Korea

[2] Good Shepherd Hosp, Seoul 135717, South Korea

来源：

BIOTECHNOLOGY LETTERS | 2005年 / 27卷 / 09期

关键词：

chondrogenesis; immunophenotype; periosteum-derived cells;

D O I：

10.1007/s10529-005-3625-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 0836 [生物工程]; 090102 [作物遗传育种]; 100705 [微生物与生化药学];

摘要：

Periosteum-derived progenitor cells (PDPCs) were isolated by characteristic surface markers. Reproducibility of immunophenotypes of the PDPCs was characterized by flow cytometric analysis using fluorescence-activated cell sorter (FACS). SH2(+), SH3(+), SH4(+), CD9(+), CD90(+) and CD105(+) were important eternal characteristic cell surface markers for the PDPCs. The characterized PDPCs maintained their chondrogenic potential in pellet cultures until the 15th passage from primary cell culture.

引用

页码：607 / 611

页数：5

共 10 条

[1]

Mesenchymal stem cell surface antigen SB-10 corresponds to activated leukocyte cell adhesion molecule and is involved in osteogenic differentiation [J].