A 'loop recapture' mechanism for ACF-dependent nucleosome remodeling

被引:73
作者
Strohner, R
Wachsmuth, M
Dachauer, K
Mazurkiewicz, J
Hochstatter, J
Rippe, K
Längst, G
机构
[1] Adolf Butenandt Inst, D-80336 Munich, Germany
[2] Kirchhoff Inst Phys, AG Mol Biophys, D-69120 Heidelberg, Germany
关键词
D O I
10.1038/nsmb966
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The ATPase ISWI is the molecular motor of several nucleosome remodeling complexes including ACF. We analyzed the ACF-nucleosome interactions and determined the characteristics of ACF-dependent nucleosome remodeling. In contrast to ISWI, ACF interacts symmetrically with DNA entry sites of the nucleosome. Two-color fluorescence cross-correlation spectroscopy measurements show that ACF can bind four DNA duplexes simultaneously in a complex that contains two Acf1 and ISWI molecules. Using bead-bound nucleosomal substrates, nucleosome movement by mechanisms involving DNA twisting was excluded. Furthermore, an ACF-dependent local detachment of DNA from the nucleosome was demonstrated in a novel assay based on the preferred intercalation of ethidium bromide to free DNA. The findings suggest a loop recapture mechanism in which ACF introduces a DNA loop at the nucleosomal entry site that propagates over the histone octamer surface and leads to nucleosome repositioning.
引用
收藏
页码:683 / 690
页数:8
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