Diversity and sharing of Haemophilus influenzae strains colonizing healthy children attending day-care centers

被引:67
作者
Farjo, RS
Foxman, B
Patel, MJ
Zhang, LX
Pettigrew, MM
McCcoy, SI
Marrs, CF
Gilsdorf, JR
机构
[1] Univ Michigan, Womens Hosp, Med Ctr, Dept Pediat & Communicable Dis, Ann Arbor, MI 48109 USA
[2] Univ Michigan, Dept Epidemiol, Ann Arbor, MI 48109 USA
关键词
Haemophilus influenzae; colonization; sharing; day care;
D O I
10.1097/01.inf.0000106981.89572.d1
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Background. Children attending day-care centers (DCCs) are at risk for Haemophilus influenzae nasopharyngeal colonization and acute otitis media. The degree to which a given strain circulates within a day-care center and the heterogeneity of strains among DCCs in a geographic area are not well-characterized. This study describes the prevalence rates of H. influenzae colonization in a large number of children attending day-care centers and. examines the genetic diversity of colonizing strains and the degree of sharing among children. Methods. Throat cultures were collected from 198 healthy children <3 years old attending 16 day-care centers in Michigan. All H. influenzae isolates were genetically typed by enterobacterial repetitive intergenic consensus PCR as the initial screening technique to identify unique strains within each child. Pulsed field gel electrophoresis was used subsequently to examine the genetic diversity of strains between children. Results. There were 127 (64%) children colonized with H. influenzae. Wide variation in rates of colonization was identified among day-care centers (0 to 95%). A total of 179 genetically unique H. influenzae strains were isolated, and 47 children (37%) were colonized with 2 or more genetically distinct H. influenzae organisms. Evidence of sharing of the same strain in different children was found in 13 of 15 colonized DCCs and 23% of genotypically unique strains were shared. Conclusion. The degree of sharing of H. influenzae among children in this study suggests transmission of these potentially pathogenic microorganisms in day-care centers.
引用
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页码:41 / 46
页数:6
相关论文
共 34 条
[1]  
Alho OP, 1996, AM J EPIDEMIOL, V143, P1149
[2]   NASOPHARYNGEAL COLONIZATION DURING THE 1ST YEAR OF LIFE [J].
ANIANSSON, G ;
ALM, B ;
ANDERSSON, B ;
LARSSON, P ;
NYLEN, O ;
PETERSON, H ;
RIGNER, P ;
SVANBORG, M ;
SVANBORG, C .
JOURNAL OF INFECTIOUS DISEASES, 1992, 165 :S38-S42
[3]   Behaviour and cognitive outcomes from middle ear disease [J].
Bennett, KE ;
Haggard, MP .
ARCHIVES OF DISEASE IN CHILDHOOD, 1999, 80 (01) :28-35
[4]   10-YEAR REVIEW OF OTITIS-MEDIA PATHOGENS [J].
BLUESTONE, CD ;
STEPHENSON, JS ;
MARTIN, LM .
PEDIATRIC INFECTIOUS DISEASE JOURNAL, 1992, 11 (08) :S7-S11
[5]  
Campos JM, 1999, MANUAL CLIN MICROBIO, P604
[6]   EVALUATION OF SAMPLING SITES FOR DETECTION OF UPPER RESPIRATORY-TRACT CARRIAGE OF STREPTOCOCCUS-PNEUMONIAE AND HAEMOPHILUS-INFLUENZAE AMONG HEALTHY FILIPINO INFANTS [J].
CAPEDING, MRZ ;
NOHYNEK, H ;
SOMBRERO, LT ;
PASCUAL, LG ;
SUNICO, ES ;
ESPARAR, GA ;
ESKO, E ;
LEINONEN, M ;
RUUTU, P .
JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (11) :3077-3079
[7]   Potential of bacterial vaccines in the prevention of acute otitis media [J].
Eskola, J ;
Kilpi, T .
PEDIATRIC INFECTIOUS DISEASE JOURNAL, 2000, 19 (05) :S72-S78
[8]   Relationship between nasopharyngeal colonization and the development of otitis media in children [J].
Faden, H ;
Duffy, L ;
Wasielewski, R ;
Wolf, J ;
Krystofik, D ;
Tung, Y .
JOURNAL OF INFECTIOUS DISEASES, 1997, 175 (06) :1440-1445
[9]   Prevalence of Haemophilus influenzae carriers in the Catalan preschool population [J].
Fontanals, D ;
Bou, R ;
Pons, I ;
Sanfeliu, I ;
Domínguez, A ;
Pineda, V ;
Renau, J ;
Muñoz, C ;
Latorre, C ;
Sanchez, F .
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 2000, 19 (04) :301-304
[10]   NASOPHARYNGEAL COLONIZATION WITH NONTYPABLE HAEMOPHILUS-INFLUENZAE AND RECURRENT OTITIS-MEDIA [J].
HARABUCHI, Y ;
FADEN, H ;
YAMANAKA, N ;
DUFFY, L ;
WOLF, J ;
KRYSTOFIK, D .
JOURNAL OF INFECTIOUS DISEASES, 1994, 170 (04) :862-866