Date palm DNA mini-preparation without liquid nitrogen

被引：13

作者：

Ouenzar, B

Hartmann, C

Rode, A

Benslimane, A

机构：

[1] Univ Cadi Ayyad, Fac Sci Semlalia, Dept Biol, Marrakech 40001, Morocco

[2] CNRS, ERS 569, Inst Biotechnol Plantes, F-91405 Orsay, France

来源：

PLANT MOLECULAR BIOLOGY REPORTER | 1998年 / 16卷 / 03期

关键词：

date palm; DNA isolation; liquid nitrogen;

D O I：

10.1023/A:1007572427029

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed a new mini-procedure for isolation of total cellular DNA from date palm (Phoenix dactylifera L.). The procedure, which does not use liquid nitrogen, has proved useful due to temporary disruptions in supplies of liquid nitrogen that occur in countries where date palm trees are cultivated. DNA suitable for RFLP and PCR analyses is obtained.

引用

页码：263 / 269

页数：7

共 13 条

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[2]

[Anonymous], PLANT MOL BIOL REPOR, DOI DOI 10.1007/BF02672010

[3]

CALLAHAN FE, 1991, PLANT MOL BIOL REP, V9, P252

[4]

Gawel N.J., 1991, PLANT MOL BIOL REP, V9, P262, DOI DOI 10.1007/BF02672076