Size fractionation of bacterial capsular polysaccharides for their use in conjugate vaccines

被引：51

作者：

Costantino, P

Norelli, F

Giannozzi, A

D'Ascenzi, S

Bartoloni, A

Kaur, S

Tang, DZ

Seid, R

Viti, S

Paffetti, R

Bigio, M

Pennatini, C

Averani, G

Guarnieri, V

Gallo, E

Ravenscroft, N

Lazzeroni, C

Rappuoli, R

Ceccarini, C

机构：

[1] Chiron Vaccines SpA, I-53100 Siena, Italy

[2] Chiron Corp, Emeryville, CA 94608 USA

来源：

VACCINE | 1999年 / 17卷 / 9-10期

关键词：

conjugate vaccines; capsular polysaccharide; oligosaccharide; intermediate chain length oligomers; hydrolysis; size fractionation;

D O I：

10.1016/S0264-410X(98)00348-X

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We have developed a chromatographic method suitable for the fractionation of polysaccharides having a negatively charged group. The method permits the removal of all those polysaccharide fragments having a short sequence and which are likely unsuitable for conjugate Vaccine construction. The selected polysaccharide fragments can be used to produce glycoconjugate vaccines containing a restricted saccharide polydispersion. We have applied this chromatographic method to three different antigens, Haemophilus influenzae type b and Neisseria meningitidis group A and group C polysaccharides. The method is easily adapted for manufacturing purposes. (C) 1999 Elsevier Science Ltd. All rights reserved.

引用

页码：1251 / 1263

页数：13

共 38 条

[1] DECLINE OF CHILDHOOD HAEMOPHILUS-INFLUENZAE TYPE-B (HIB) DISEASE IN THE HIB VACCINE ERA [J].