Nitric oxide modulates gut ischemia-reperfusion-induced P-selectin expression in murine liver

被引:30
作者
Horie, Y
Wolf, R
Anderson, DC
Granger, DN
机构
[1] Louisiana State Univ, Med Ctr, Dept Cellular & Mol Physiol, Shreveport, LA 71130 USA
[2] Louisiana State Univ, Med Ctr, Ctr Excellence Arthrit & Rheumatol, Dept Med, Shreveport, LA 71130 USA
[3] Pharmacia & Upjohn Inc, Labs, Discovery Res, Kalamazoo, MI 49001 USA
来源
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY | 1998年 / 275卷 / 02期
关键词
leukocyte endothelial cell adhesion; nitric oxide synthase; lung; L-arginine;
D O I
10.1152/ajpheart.1998.275.2.H520
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Reperfusion of ischemic intestine is associated with P-selectin-dependent adhesion of leukocytes in the liver microcirculation. The objective of this study was to define the contribution of nitric oxide (NO) to the enhanced P-selectin expression and increased leukocyte rolling elicited by gut ischemia-reperfusion (I/R). Leukocyte rolling (monitored by intravital microscopy) in the terminal hepatic venules (THV) and P-selectin expression (measured using the dual-radiolabeled monoclonal antibody technique) were determined in mice after 15 min of superior mesenteric artery occlusion and 30 min of reperfusion. After 30 min of reperfusion, P-selectin expression was significantly increased in the liver, intestine, and lung, with a corresponding increase in the number of rolling leukocytes (in THV). The NO synthase inhibitor NG-monomethyl-L-arginine exaggerated the gut YR-induced increases in both rolling leukocytes and P-selectin expression (in liver and intestine), responses that were not detected with coadministration of L-arginine or in P-selectin-deficient mice. The NO donor diethylenetriamine/NO and L-arginine were both effective in attenuating the gut I/R-induced increases in rolling leukocytes (in THV) and P-selectin expression (in liver, intestine, and lung). These findings indicate that NO modulates gut I/R-induced recruitment of rolling leukocytes in THV via an action on P-selectin expression.
引用
收藏
页码:H520 / H526
页数:7
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