Detection of Norwalk-like virus genes in the caecum contents of pigs

被引：121

作者：

Sugieda, M

Nagaoka, H

Kakishima, Y

Ohshita, T

Nakamura, S

Nakajima, S

机构：

[1] Natl Inst Publ Hlth, Dept Microbiol, Minato Ku, Tokyo 1088638, Japan

[2] Shizuoka Prefectural Inst Publ Hlth & Environm Sc, Shizuoka, Japan

来源：

ARCHIVES OF VIROLOGY | 1998年 / 143卷 / 06期

关键词：

D O I：

10.1007/s007050050369

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Norwalk-like virus genes were detected by RT-PCR in the caecum contents of pigs. Positive PCR products were produced from four out of 1117 samples by nested PCR using human SRSV primers. Nucleotide sequences between 4561 and 4852 numbered according to the Norwalk virus genomic RNA in the RNA polymerase region were determined. Between the Norwalk virus sequence and the sequences detected in pigs, there was 58.2% to 59.9% sequence homology. The swine sequences were located on genogroup II of human SRSVs, but formed a subgroup in the phylogenetic tree of caliciviruses.

引用

页码：1215 / 1221

页数：7

共 19 条

[1] RELATIONSHIPS AMONG THE POSITIVE STRAND AND DOUBLE-STRAND RNA VIRUSES AS VIEWED THROUGH THEIR RNA-DEPENDENT RNA-POLYMERASES [J].