Regulated expression of the Escherichia coli dam gene

被引：14

作者：

Calmann, MA ^{[1
]}

Marinus, MG ^{[1
]}

机构：

[1] Univ Massachusetts, Sch Med, Dept Biochem & Mol Pharmacol, Worcester, MA 01655 USA

来源：

JOURNAL OF BACTERIOLOGY | 2003年 / 185卷 / 16期

关键词：

D O I：

10.1128/JB.185.16.5012-5014.2003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Regulated expression of the Escherichia coli dam gene has been achieved with the araBAD promoter lacking a ribosome binding site. Cultures of dam mutants containing plasmid pMQ430 show no detectable methylation in the absence of arabinose and complete methylation in its presence. Dam methyltransferase is a substrate for the Lon protease.

引用

页码：5012 / 5014

页数：3

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