Identification of PaPKS1, a polyketide synthase involved in melanin formation and its use as a genetic tool in Podospora anserina

被引：32

作者：

Coppin, Evelyne

Silar, Philippe

机构：

[1] Univ Paris 11, Inst Genet & Microbiol, CNRS, UMR 8621, F-91405 Orsay, France

[2] Univ Paris 07, UFR Biochim, F-75005 Paris, France

来源：

MYCOLOGICAL RESEARCH | 2007年 / 111卷

关键词：

melanin; Podospora anserina; polyketide synthase; RIP;

D O I：

10.1016/j.mycres.2007.05.011

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

In the filamentous fungus Podospora anserina, many pigmentation mutations map to the median region of the complex locus '14', called segment '29'. The data presented in this paper show that segment 29 corresponds to a gene encoding a polyketide synthase, designated PaPKS1, and identifies two mutations that completely or partially abolish the activity of the PaPKS1 polypeptide. We present evidence that the P. anserina green pigment is a (DHN)-melanin. Using the powerful genetic system of PaPKS1 cloning, we demonstrate that in P. anserina trans-duplicated sequences are subject to the RIP process as previously demonstrated for the cis-duplicated regions. (C) 2007 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.

引用

收藏

页码：901 / 908

页数：8

相关论文

共 30 条

[1] BASIC LOCAL ALIGNMENT SEARCH TOOL [J].

ALTSCHUL, SF ;

GISH, W ;

MILLER, W ;

MYERS, EW ;

LIPMAN, DJ .

JOURNAL OF MOLECULAR BIOLOGY, 1990, 215 (03) :403-410

[2] Altering a gene involved in nuclear distribution increases the repeat-induced point mutation process in the fungus Podospora anserina [J].

Bouhouche, K ;

Zickler, D ;

Debuchy, R ;

Arnaise, S .

GENETICS, 2004, 167 (01) :151-159

[3] TRANSFORMATION BY INTEGRATION IN PODOSPORA-ANSERINA .1. METHODOLOGY AND PHENOMENOLOGY [J].

BRYGOO, Y ;

DEBUCHY, R .

MOLECULAR & GENERAL GENETICS, 1985, 200 (01) :128-131

[4] Characterization of two polyketide synthase genes in Exophiala lecanii-corni, a melanized fungus with bioremediation potential [J].

Cheng, Q ;

Kinney, KA ;

Whitman, CP ;

Szaniszlo, PJ .

BIOORGANIC CHEMISTRY, 2004, 32 (02) :92-108

[5] Molecular cloning and characterization of WdPKS1, a gene involved in dihydroxynaphthalene melanin biosynthesis and virulence in Wangiella (Exophiala) dermatitidis [J].

Feng, B ;

Wang, X ;

Hauser, M ;

Kaufmann, S ;

Jentsch, S ;

Haase, G ;

Becker, JM ;

Szaniszlo, PJ .

INFECTION AND IMMUNITY, 2001, 69 (03) :1781-1794

[6] A melanin polyketide synthase (PKS) gene from Nodulisporium sp that shows homology to the pks1 gene of Colletotrichum lagenarium [J].

Fulton, TR ;

Ibrahim, N ;

Losada, MC ;

Grzegorski, D ;

Tkacz, JS .

MOLECULAR AND GENERAL GENETICS, 1999, 262 (4-5) :714-720

[7] RIP: the evolutionary cost of genome defense [J].

Galagan, JE ;

Selker, EU .

TRENDS IN GENETICS, 2004, 20 (09) :417-423

[8] Genome quality control:: RIP (repeat-induced point mutation) comes to Podospora [J].

Graïa, F ;

Lespinet, O ;

Rimbault, B ;

Dequard-Chablat, M ;

Coppin, E ;

Picard, M .

MOLECULAR MICROBIOLOGY, 2001, 40 (03) :586-595

[9] The degenerate DNA transposon Pat and repeat-induced point mutation (RIP) in Podospora anserina [J].

Hamann, A ;

Feller, F ;

Osiewacz, HD .

MOLECULAR AND GENERAL GENETICS, 2000, 263 (06) :1061-1069

[10] Phylogenomic analysis of type I polyketide synthase genes in pathogenic and saprobic ascomycetes [J].

Kroken, S ;

Glass, NL ;

Taylor, JW ;

Yoder, OC ;

Turgeon, BG .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (26) :15670-15675