Identification of PaPKS1, a polyketide synthase involved in melanin formation and its use as a genetic tool in Podospora anserina

被引：31

作者：

Coppin, Evelyne

Silar, Philippe

机构：

[1] Univ Paris 11, Inst Genet & Microbiol, CNRS, UMR 8621, F-91405 Orsay, France

[2] Univ Paris 07, UFR Biochim, F-75005 Paris, France

来源：

MYCOLOGICAL RESEARCH | 2007年 / 111卷

关键词：

melanin; Podospora anserina; polyketide synthase; RIP;

D O I：

10.1016/j.mycres.2007.05.011

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

In the filamentous fungus Podospora anserina, many pigmentation mutations map to the median region of the complex locus '14', called segment '29'. The data presented in this paper show that segment 29 corresponds to a gene encoding a polyketide synthase, designated PaPKS1, and identifies two mutations that completely or partially abolish the activity of the PaPKS1 polypeptide. We present evidence that the P. anserina green pigment is a (DHN)-melanin. Using the powerful genetic system of PaPKS1 cloning, we demonstrate that in P. anserina trans-duplicated sequences are subject to the RIP process as previously demonstrated for the cis-duplicated regions. (C) 2007 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.

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收藏

页码：901 / 908

页数：8

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