Tissue engineered bone: Measurement of nutrient transport in three-dimensional matrices

被引:144
作者
Botchwey, EA
Dupree, MA
Pollack, SR
Levine, EM
Laurencin, CT
机构
[1] Univ Virginia, Dept Biomed Engn, Charlottesville, VA 22903 USA
[2] Univ Virginia, Dept Orthopaed Surg, Charlottesville, VA 22903 USA
[3] Univ Penn, Dept Bioengn, Philadelphia, PA 19104 USA
[4] Wistar Inst Anat & Biol, Philadelphia, PA 19104 USA
[5] Univ Virginia, Dept Chem Engn, Charlottesville, VA 22903 USA
来源
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A | 2003年 / 67A卷 / 01期
关键词
tissue engineering; scaffold; bone; diffusion; flow;
D O I
10.1002/jbm.a.10111
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
The classic paradigm for in vitro tissue engineering of bone involves the isolation and culture of donor osteoblasts or osteoprogenitor cells within three-dimensional (3D) scaffold biomaterials under conditions that support tissue growth and mineralized osteoid formation. Our studies focus on the development and utilization of new dynamic culture technologies to provide adequate nutrient flux within 3D scaffolds to support ongoing tissue formation. In this study, we have developed a basic one-dimensional (1D) model to characterize the efficiency of passive nutrient diffusion and transport flux to bone cells within 3D scaffolds under static and dynamic culture conditions. Internal fluid perfusion within modeled scaffolds increased rapidly with increasing pore volume and pore diameter to a maximum of approximately 1% of external fluid flow. In contrast, internal perfusion decreased significantly with in-creasing pore channel tortuosity. Calculations of associated nutrient flux indicate that static 3D culture and some inappropriately designed dynamic culture environments lead to regions of insufficient nutrient concentration to maintain cell viability, and can result in steep nutrient concentration gradients within the modeled constructs. These quantitative studies provide a basis for development of new dynamic culture methodologies to overcome the limitations of passive nutrient diffusion in 3D cell-scaffold composite systems proposed for in vitro tissue engineering of bone. (C) 2003 Wiley Periodicals, Inc.
引用
收藏
页码:357 / 367
页数:11
相关论文
共 38 条
[1]   Serum modulates the intracellular calcium response of primary cultured bone cells to shear flow [J].
Allen, FD ;
Hung, CT ;
Pollack, SR ;
Brighton, CT .
JOURNAL OF BIOMECHANICS, 2000, 33 (12) :1585-1591
[2]  
ALLEN FD, 1997, T ORTHOPAEDIC RES SO, V22, P706
[3]  
Attawia MA, 1999, J BIOMED MATER RES, V48, P322, DOI 10.1002/(SICI)1097-4636(1999)48:3<322::AID-JBM17>3.0.CO
[4]  
2-U
[5]   OSTEOBLAST-LIKE CELL ADHERANCE AND MIGRATION THROUGH 3-DIMENSIONAL POROUS POLYMER MATRICES [J].
ATTAWIA, MA ;
HERBERT, KM ;
LAURENCIN, CT .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1995, 213 (02) :639-644
[6]  
ATTAWIA MA, 1994, ABSTR PAP AM CHEM S, V2086, P278
[7]   Tissue engineered microsphere-based matrices for bone repair: design and evaluation [J].
Borden, M ;
Attawia, M ;
Khan, Y ;
Laurencin, CT .
BIOMATERIALS, 2002, 23 (02) :551-559
[8]  
Botchwey EA, 2003, BIORHEOLOGY, V40, P299
[9]  
Botchwey EA, 2001, J BIOMED MATER RES, V55, P242
[10]  
BURWELL BG, 1994, BONE GRAFTS DERIVATI, p3P