Low frequency of T-DNA based activation tagging in Arabidopsis is correlated with methylation of CaMV 35S enhancer sequences

被引：23

作者：

Chalfun, A

Mes, JJ

Mlynárová, L

Aarts, MGM

Angenent, GC

机构：

[1] Plant Res Int, Business Unit Biosci, NL-6708 PD Wageningen, Netherlands

[2] Wageningen Univ, Genet Lab, NL-6703 BD Wageningen, Netherlands

来源：

FEBS LETTERS | 2003年 / 555卷 / 03期

关键词：

activation tagging; Arabidopsis mutant; transcriptional gene silencing; DNA methylation;

D O I：

10.1016/S0014-5793(03)01300-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A powerful system to create gain-of-function mutants in plants is activation tagging using T-DNA based vehicles to introduce transcriptional enhancer sequences. Large Arabidopsis populations of individual plants carrying a quadruple cauliflower mosaic virus (CaMV) 35S enhancer are frequently used for mutant screenings, however the frequency of morphological mutants remains very low. To clarify this low frequency we analyzed a subset of lines generated by this method. The correlation between the number of T-DNA insertion sites, the methylation status of the 35S enhancer sequence and 35S enhancer activity was determined. All plants containing more than a single TDNA insertion showed methylation of the 35S enhancer and revealed a dramatic decrease in 35S enhancer activity. The results support the notion that in a large proportion of the TDNA based activation tagged lines the 35S transcriptional enhancer is silenced due to methylation, which is induced by multiple T-DNA integrations. (C) 2003 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：459 / 463

页数：5

共 34 条

[1] Activation tagging identifies a conserved MYB regulator of phenylpropanoid biosynthesis [J].