Generation of antibodies against prion protein in wild-type mice via helix 1 peptide immunization

被引：26

作者：

Arbel, M ^{[1
]}

Lavie, V ^{[1
]}

Solomon, B ^{[1
]}

机构：

[1] Tel Aviv Univ, George S Wise Fac Life Sci, Dept Mol Microbiol & Biotechnol, IL-69978 Tel Aviv, Israel

来源：

JOURNAL OF NEUROIMMUNOLOGY | 2003年 / 144卷 / 1-2期

关键词：

prion protein; active immunization; anti-PrP antibodies; helix; 1;

D O I：

10.1016/j.jneuroim.2003.08.036

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We present here the development of antibodies against prion protein in BALB/C mice using as antigen human helix I of PrP. This sequence is suggested to be involved in protein pathological conformational changes, and is distinguished from that of mice by one amino acid. The immune tolerance to an 'almost-self' epitope and the poor immunogenicity of short peptides was overcome by using Multiple Antigen Peptide displaying eight copies of helix 1. The generated antibodies recognize the whole prion protein with a high binding constant and the established protocol may lead to an active immunization towards therapeutics of prion disease. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：38 / 45

页数：8

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