Comparison of simple and rapid methods for identifying enterococci intrinsically resistant to vancomycin

被引:13
作者
Hanson, KL
Cartwright, CP
机构
[1] Hennepin Cty Med Ctr, Clin Labs, Dept Microbiol, Minneapolis, MN 55415 USA
[2] Univ Minnesota, Sch Med, Dept Lab Med & Pathol, Minneapolis, MN 55455 USA
关键词
D O I
10.1128/JCM.37.3.815-817.1999
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Three different methodologies, reduction of litmus milk (LM) and acidification of arabinose (ARA), acidification of methyl-alpha-D-glucopyranoside (MGP), and rapid motility (RM), for differentiating isolates of Enterococcus casseliflavus and Enterococcus gallinarum (intrinsically vancomycin-resistant enterococci [IVRE]) from Enterococcus faecalis and Enterococcus faecium were evaluated. All 33 isolates of E. faecalis tested reduced LM within 4 h and were negative in all other tests, while the 53 isolates of E. faecium were ARA positive only. In contrast, 45 of 46 (98%) IVRE isolates examined (26 E. casseliflavus and 20 E. gallinarum isolates) acidified MGP, 41 of 46 (89%) were LM and ARA positive, and 45 of 46 (98%) were RM positive. Acidification of MGP was therefore the single most useful test for differentiating IVRE from vancomycin-resistant E. faecium and E. faecalis; however, a combination of LM-ARA and RM testing enabled the correct designation of organisms without the need for overnight incubation.
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页码:815 / 817
页数:3
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